1999 Fiscal Year Final Research Report Summary
An organ-specific autoantigen for Sjogren's syndrome induced by Epstein-Barr virus activation
| Project/Area Number |
10470381
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
SAITO Ichiro The University of Tokushima, School of Dentistry, Associate Professor, 歯学部, 助教授 (60147634)
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| Co-Investigator(Kenkyū-buntansha) |
TSUBOTA Kazuo Tokyo Dental collage, Department of Ophthalmology, Professor, 眼科学, 教授 (40163878)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Sjogren's syndrome / Epstein-Barr virus / autoantigen / virus reactivation / apoptosis |
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| Research Abstract |
Sjogren's syndrome (SS) is an organ-specific autoimmune disease characterized by exocrine gland destruction, and Epstein-Barr virus (EBV) has been implicated in the pathogenesis of SS. The 120-kD a-fodrin may be a specific autoantigen in the pathogenesis of SS, but the mechanisms of a-fodrin cleavage leading to autoantigen formation remain unclear. A striking correlation between antibodies against 120-kD a-fodrin and a reactivated EBV antigen was found in sera from patients with SS, but not in those from normal individuals. To study whether EBV activation could induce the 120-kD a-fodrin, Akata cells were analyzed by immunoblot method. EBV-activated Akata cells had specific a-fodrin cleavage to the expected 120-kD fragments. Furthermore, caspase inhibitors inhibited the 120-kD a-fodrin cleavage. Thus, these results indicate that EBV reactivation may play an important role in the progression of a-fodrin cleavage by induction of apoptotic protease activities and the development of SS.
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Research Products
(12 results)
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[Publications] Yanagi, K., Ishimaru, N., Haneji, N., Saegusa, K., Saito, I., Hayashi, Y.: "Anti-120-kDa a-fodrin immune response with Th1-cytokine profile in the NOD mouse model of Sjogren's syndrome."Eur. J. Immunol.. 28. 3336-3345 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Saito, I., Haruta, K., Shimuta, †M., Inoue, H., Sakurai, H., Yamada, K., Ishimaru, N., Higashiyama, H., Sumida, T, Ishida, H., Suda, T., Noda,T., Hayashi, Y., Tsubota, K.: "+Fas ligand-mediated exocrinopathy resembling Sjogren's syndrome in mice transgenic for IL-10."J. Immunol.. 162. 2488-2494 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Tsubota, K., Goto, E., Fujita, H., Ono, M., Inoue, H., Saito, I., Shimmura, S.: "Treatment of dry eye by autologous serum application in Sjogren's syndrome."Br. J. Ophtalmol.. 83. 390-395 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Ishimaru, N., Saegusa, K., Yanagi,K., Haneji, N., Saito, I., Hayashi, Y.: "Estrogen deficiency accelerate autoimmune exocrinopathy in murine Sjogren's syndrome through Fas-mediated apoptosis."Am. J. Pathol.. 155. 173-181 (1999)
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「研究成果報告書概要(欧文)」より
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[Publications] Horiuchi, M., Yamano, S., Inoue, H, Ishii, J., Nagata, Y., Adachi, H., Ono, M., Renard, J.N., Mizuno, F., Hayashi, Y., Saito, I.: "Possible involvement of IL-12 expression by Epstein-Barr virus in Sjogren syndrome."J. Clin. Pathol.. 52. 833-837 (1999)
Description
「研究成果報告書概要(欧文)」より
