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2000 Fiscal Year Final Research Report Summary

Activin signal transduction and its regulation mechanisms

Research Project

Project/Area Number 10480170
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionUniversity of Tokushima

Principal Investigator

SUGINO Hiromu  University of Tokushima, The Institute for Enzyme Research, Professor, 分子酵素学研究センター, 教授 (50211305)

Co-Investigator(Kenkyū-buntansha) OGURA Yasunori  University of Tokushima, The Institute for Enzyme Research, Research Associate, 分子酵素学研究センター, 助手 (60304557)
NAKAMURA Takanori  Kagawa Medical University, Professor, 医学部, 教授 (70183887)
TSUCHIDA Kunihiro  University of Tokushima, The Institute for Enzyme Research, Associate Professor, 分子酵素学研究センター, 助教授 (30281091)
SUGINO Hiromu  University of Tokushima, The Institute for Enzyme Research, Professor (50211305)
Project Period (FY) 1998 – 2000
Keywordsactivin / follistatin / ARIP / PDZ domain / WW domain / FLRG / FLRG
Research Abstract

In this term, we have identified novel regulatory proteins that control activin functions either intracellularly or extracellularly.
(1) Intracellular control
We have identified a mouse PDZ protein that interacts with the activin type IIA receptor (ActRIIA), which we named activin receptor-interacting protein 1 (ARIP1). By using yeast two-hybrid screening, we isolated a cDNA clone of ARIP1 from a mouse brain cDNA library. ARIP1 had one guanylate kinase domain in the NH_2-terminal region, followed by two WW domains and five PDZ domains (PDZ-1-5). ARIP1-short had a deletion in the NH_2-terminal region and lacked the guanylate kinase domain. Both forms interacted with ActRIIA through PDZ5. ARIP1 interacted specifically with ActRIIA among the receptors for the transforming growth factor β family. Interestingly, ARIP1 also interacted with Smad3. The mRNA of ARIP1 was more abundant in the brain than in other tissues. Overexpression of ARIP1 controled activin-induced and Smad3-induced transcrip … More tion in activin-responsive cell lines. These findings suggest that ARIP1 has a significant role in assembling activin signaling molecules at specific subcellular sited and in regulating signal transduction in neuronal cells.
(2) Extracellular control
Follistatin is an activin-binding protein that prevents activin from binding to its receptors and neutralizes its activity. Follistatin also binds bone morphogenetic proteins (BMPs). In this study, we identified a novel follistatin-like protein from mouse. The mouse cDNA encoded most likely a mouse homologue of human FLRG.Whereas follistatin has three follistatin domains, FLRG possessed only two follistatin domains. Like follistatin, FLRG had higher affinity for activin than for BMP-2. The FLRG protein inhibited activin-induced and BMP-2-induced transcriptional responses in a dose-dependent manner. Ligand blotting using ^<125>I-activin revealed that the COOH-terminal region containing the second follistatin domain was able to bind activin. Our finding implies that cellular signaling by activin and BMPs is tightly regulated by multiple members of the follistatin family. Less

  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Yamane,Y. et al.: "Molecular characterization of Xenopus embryo heparan sulfate : Differential structural requirements for the specific binding to basic fibroblast growth factor and follistatin."J.Biol.Chem.. 273. 7375-7381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaoka,T. et al.: "Hypoplasia of pancreatic islets in transgenic mice expressing activin receptor mutants."J.Clin.Invest.. 102. 294-301 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iemura,S. et al.: "Direct binding of follistatin to a complex of bone morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo."Proc.Natl.Acad.Sci.U.S.A.. 95. 9337-9342 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shoji,H. et al.: "Identification of a novel type II activin receptor, type IIA-N, induced during the neural differentiation of murine P19 embryonal carcinoma cells."Biochem.Biophys.Res.Commun.. 246. 320-324 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohguchi,M. et al.: "Activin A regulates the production of mature interleukin-1β and interleukin-1 receptor antagonist in human monocytic cells."J.Inter.Cyto.Res.. 18. 491-498 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto,O. et al.: "The role of activin type I receptors in activin A-induced growth arrest and apoptosis in mouse B cell hybridoma cells."Cell.Signal.. 10. 743-749 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Okabayashi,K. et al.: "cDNA cloning and distribution of the Xenopus follistatin-related protein."Biochem.Biophys.Res.Commun.. 254. 42-48 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishisaki,A. et al.: "Differential inhibition of Smad6 and Smad7 on bone morphogenetic protein- and activin-mediated growth arrest and apoptosis in B cells."J.Biol.Chem.. 274. 13637-13642 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyamoto,S. et al.: "Effects of activin on hormone secretion by single female rat pituitary cells : Analysis by cell immunoblot aasay."J.Endocrinol.. 161. 375-382 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shoji,H. et al.: "Identification and characterization of a PDZ protein that interacts with activin type II receptors."J.Biol.Chem.. 275. 5485-5492 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamura,N. et al.: "Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells."Eur.J.Endocrinol.. 142. 506-511 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sumitomo,K. et al.: "Expression of a TCF-β1 inducible gene, TSC-36, causes growth inhibition in human lung cancer cell line."Cancer Lett.. 155. 37-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoh,M. et al.: "Activin promotes astrocytic differentiation of a multipotent neural stem cell line and an astrocyte progenitor cell line from murine central nervous system. "Neurosci.Lett.. 284. 143-146 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Florio,P. et al.: "Activin A stimulates insulin secretion in cultured human pancreatic islets."J.Endocrinol.Invest.. 23. 231-234 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] de Groot,E. et al.: "Expression patterns of follistatin and two follistatin-related proteins during mouse development."Int.J.Dev.Biol.. 44. 327-330 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto,O. et al.: "Differentiation between follistatin isoforms in the inhibition of activin signalling : Activin neutralizing activity of follistatin isoforms is dependent on their affinity for activin."Cellular Signalling. 12. 565-571 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsuchida,K. et al.: "Identification and characterizaiton of a novel follistatin-like protein as a binding protein for the TCF-β family."J.Biol.Chem.. 275. 40788-40796 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉野弘,土田邦博: "サイトカイン・増殖因子"羊土社. 2 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 土田邦博,杉野弘: "サイトカイン・増殖因子"羊土社. 2 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉野弘,土田邦博: "サイトカイン・増殖因子"羊土社. 1 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 杉野弘: "医学のあゆみ"医歯薬出版株式会社. 4 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 土田邦博: "生化学"日本生化学会. 6 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sugino,H. et al.: "Activin and Follistatin. In Skeletal Growth Factors"Lippincott Williams & Willkins, Philadelphia.. 13 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamane, Y.et al.: "Molecular characterization of Xenopus embryo heparan sulfate : Differential structural requirements for the specific binding to basic fibroblast growth factor and follistatin."J.Biol.Chem.. 273. 7375-7381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaoka, T.et al.: "Hypoplasia of pancreatic islets in transgenic mice expressing activin receptor mutants."J.Clin.Invest.. 102. 294-301 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iemura, S.et al.: "Direct binding of follistatin to a complex of bone morphogenetic protein and its receptor inhibits ventral and epidermal cell fates in early Xenopus embryo."Proc.Natl.Acad.Sci.U.S.A.. 95. 9337-9342 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shoji, H.et al.: "Identification of a novel type II activin receptor, type IIA-N, induced during the neural differentiation of murine P19 embryonal carcinoma cells."Biochem, Biophys.Res.Commun.. 246. 320-324 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohguchi, M.et al.: "Activin A regulates the production of mature interleukin-1β and interleukin-1 receptor antagonist in human monocytic cells."J.Inter.Cyto.Res.. 18. 491-498 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, O.et al.: "The role of activin type I receptors in activin A-induced growth arrest and apoptosis in mouse B cell hybridoma cells."Cell.Signal.. 10. 743-749 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okabayashi, K.et al.: "cDNA cloning and distribution of the Xenopus follistatin-related protein."Biochem.Biophys.Res.Commun.. 254. 42-48 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishisaki, A.et al.: "Differential inhibition of Smad6 and Smad7 on bone morphogenetic protein- and activin-mediated growth arrest and apoptosis in B cells."J.Biol.Chem.. 274. 13637-13642 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyamoto, S.et al.: "Effects of activin on hormone secretion by single female rat pituitary cells : analysis by cell immunoblot aasay."J.Endocrinol.. 161. 375-382 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shoji, H.et al.: "Identification and characterization of a PDZ protein that interacts with activin type II receptors."J.Biol.Chem.. 275. 5485-5492 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamura, N.et al.: "Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells."Eur.J.Endocrinol.. 142. 506-511 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sumitomo, K.et al.: "Expression of a TGF-β1 inducible gene, TSC-36, causes growth inhibition in human lung cancer cell line."Cancer Lett.. 155. 37-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, M.et al.: "Activin promotes astrocytic differentiation of a multipotent neural stem cell line and an astrocyte progenitor cell line from murine central nervous system."Neurosci.Lett.. 284. 143-146 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Florio, P.et al.: "Activin A stimulates insulin secretion in cultured human pancreatic islets."J.Endocrinol.Invest.. 23. 231-234 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] de Groot, E.et al.: "Expression patterns of follistatin and two follistatin-related proteins during mouse development."Int.J.Dev.Biol.. 44. 327-330 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, O.et al.: "Differentiation between follistatin isoforms in the inhibition of activin signalling : Activin neutralizing activity of follistatin isoforms is dependent on their affinity for activin."Cellular Signalling. 12. 565-571 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchida, K.et al.: "Identification and characterizaiton of a novel follistatin-like protein as a binding protein for the TGF-β family."J.Biol.Chem.. 275. 40788-40796 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino, H.et al.: "Activin and inhibin"Cytokines and Growth Factors. 148-149 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchida, K.et al.: "Activin receptors (ActR)"Cytokines and Growth Factors. 150-151 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino, H.et al.: "Follistatin (FS)"Cytokines and Growth Factors. 154 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino, H.: "Follistatin - Its molecular heterogeneity and regulation of activin action"J.Clin.Expe.Med.. 185. 169-172 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuchida, K.: "Signal transduction through receptor serine/threonine kinase and its regulation."J.Med.. 70. 1430-1435 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sugino, H.et al.: "Activin and follistatin"Skeletal Growth Factors (Canalis, E.ed). 251-262 (2000)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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