1999 Fiscal Year Final Research Report Summary
New methods for expression cloning of alternative splicing factors
| Project/Area Number |
10558102
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | TOKYO MEDICAL & DENTAL UNIVERSITY |
|---|
Principal Investigator |
HAGIWARA Masatoshi MEDICAL RESEARCH INSTITUTE, TOKYO MEDICAL & DENTAL UNIVERSITY, PROFESSOR, 難治疾患研究所, 教授 (10208423)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURO Yoshinao NAGOYAMEDICAL UNIVERSITY-ASSISTANT PROFESSOR, 医学部, 助教授 (80270990)
|
|---|
| Project Period (FY) |
1998 – 1999
|
| Keywords | alternative splicing / GFP / arginine methylation / RNAi / SRPK / phosphorylation of SR proteins |
|---|
| Research Abstract |
We tried to establish a new method to screen RNA splicing factors using a calcitonin/CGRP mini gene with GFP expression vector. Although we had expected that regulators for neuron-specific alternative splicing could be cloned with this system, no change of GFP expression was observed in any cells used for this experiment. However, we succeeded to get clones of potential RNA-binding proteins with another method which we originally developed.
|
Research Products
(12 results)
-
-
-
-
-
-
-
[Publications] Nagai, Y., Miyazaki, M., Aoki, R., Zama, T., Inoue, S., Hirose, K., Iino, M., and Hagiwara, M.: "Detection of cAMP-induced phosphorylation with a novel fluorescent indicator."Nat.Biotechnol.. 18. 313-316 (2000)
Description
「研究成果報告書概要(欧文)」より
-
-
[Publications] Koizumi, J., Okamoto, Y., Onogi, H., Mayeda, A., and Krainer, A., and Hagiwara, M.: "The subcellular localization of SF2/ASF is regulated by the direct interaction with SR protein kinases (SRPKs)."J.Biol.Chem.. 274. 11125-11131 (1999)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Okamoto, Y., Onogi, H., Honda, R., Yasuda, H., Wakabayashi, T., Nimura, Y., and Hagiwara, M.: "cdc2 kinasa-mediated phosporylation of splicing factor. SF2/ASF."Biochemical and Biophysical Research Communications.. 249. 872-878 (1998)
Description
「研究成果報告書概要(欧文)」より
-
-
