1999 Fiscal Year Final Research Report Summary
Fas antigen and Fas ligand with Oral Disease
| Project/Area Number |
10671900
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kyushu Dental College |
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Principal Investigator |
FUKUDA Jinichi Kyushu Dental College, Professor, 歯学部, 教授 (00106270)
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| Co-Investigator(Kenkyū-buntansha) |
HANEJI Tatsuji Tokushima University, Professor, 歯学部, 教授 (50156379)
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| Project Period (FY) |
1998 – 1999
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| Keywords | Fas antigen / Fas ligand / apoptosis / oral cancer |
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| Research Abstract |
To determine the relationship between Fas antigen and apoptosis in oral epithelium, we investigated the expression of Fas antigen in oral mucosa by immunohistochemical and immunoblotting methods.
Fas antigen distributed in stratum spinosum and basal part of the stratum coreneum in normal human gingiva. The estimated molecular weight of Fas antigen was calculated as 35,000, which consistent with the value reported for the Fas antigen. Expression of Fas antigen was related to the degree of the tumor differentiation.
Using RT-PCR, messenger RNA for Fas antigen was detected in the human oral squamous cell carcinoma cell line, SCC-25. In serum-free medium, a monoclonal ante-Fas antibody induced expression of Fas antigen in SCC-25 cells. Fas antigen was localized to the cytoplasm and the cell membrane. The molecular weight of the protein recognized was 35,000. Anti-Fas monoclonal antibody induced cytotoxicity in SCC-25 cells in a time-dependent manner up to 8 h. Marked nuclear condensation and fragmentation of chromatin were observed in the anti-Fas monoclonal antibody-treated cells using Hoechst 33342 staining. This antibody also induced DNA ladder formation in SCC-25 cells in a time-dependent manner. The present results indicate that the anti Fas monoclonal antibody may mediate apoptosis by binding to the Fas antigen expressed in SCC-cells.
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