Chloride concentration in cultured hippocampal neurons increases during long-term exposure to ammonia through enhanced expression of an anion exchanger

1999 Fiscal Year Final Research Report Summary

• Project/Area Number: 10672163
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 応用薬理学・医療系薬学
• Research Institution: Kansai Medical University
• Principal Investigator: HATTORI Naoki Kansai Medical University, Department of Pharmacology, Assistant Professor, 医学部, 講師 (80288828)
• Co-Investigator(Kenkyū-buntansha): KITAGAWA Kaori Kansai Medical University, Department of Pharmacology, Research Associate, 医学部, 助手 (10165813)
• Project Period (FY): 1998 – 1999
• Keywords: chloride ion / brain / ammonia / anion exchanger / protein kinase C

Research Abstract

Mechanisms of ammonia-induced increases in neuronal [ClィイD1=ィエD1]i
A neurotoxic factor in hepatic encephalopathy, ammonia (2 mM, 48 hr), increased [ClィイD1-ィエD1]i in cultured rat hippocampal neurons. The increase was found to be due to enhanced expression of anion exchanger (AE3) mediated by protein kinase C activation.

Research Products

• [Publications] Irie, T et al.: "Chloride concentration in cultured hippocampal neurons increases during long-term exposure to ammonia through expression of anion exchanger"Brain Res.. 806. 246-256 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hattori, N et al.: "Cl^--ATPase and Na^+, K^+-ATPase activities in Alzheimer's disease brains"Neurosci. Lett. 254. 141-144 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Zeng, X.-T et al.: "Antiserum against Cl^- pump complex recognized 51 kDa protein, a possible catalytic unit in the rat brain"Neurosci. Lett. 258. 85-88 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Irie,T., Hara, M, Yasukura, T., Minamino, M., Omori, K., Matsuda, H., Inoue, K. and Inagaki, C.: "Chloride concentration in cultured hippocampal neurons increases during long-term exposure to ammonia through expression of anion exchanger."Brain Res. 806. 246-256 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori, N., Kitagawa, K., Higashida, T., Yagyu, K., Shimohama, S., Takaya, T., Perry, G., Smith, M. A. and Inagaki, C.: "ClィイD1-ィエD1-ATPase and NaィイD1+ィエD1, KィイD1+ィエD1-ATPase activities in Alzheimer's disease brains."Neurosci. Lett.. 254. 141-144 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori, N. and Inagaki, C.: "Immunological aspects of human growth hormone and prolactin."Domestic Animal Endocrynology. 15. 371-375 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Zeng, X.-T., Higashida, T., Hara, M., Hattori, N., Kitagawa, K., Omori, K. and Inagaki, C.: "Antiserum against ClィイD1-ィエD1 pump complex recognized 51 kDa protein, a possible catalytic unit in the rat brain."Neurosci. Lett.. 258. 85-88 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kawasaki, E., Hattori, N., Miyamoto, E., Yamashita, T. and Inagaki, C.: "Single-cell RT-PCR demonstrates expression of voltage-dependent chloride channels (ClC-1, ClC-2 and ClC-3) in outer hair cells of rat cochlea."Brain Res. 838. 166-170 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hattori, N., Kaori Kitagawa and Chiyoko Inagaki: "Human lymphocytes express hGH-N gene transcripts of 22kDa, 20kDa and minor forms of GH, but not hGH-V gene."Eur. J. Endocrinol. 141. 413-418 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yagyu, K., Kitagawa, K., Irie, T., Hattori, N., Omori, K., Inagaki, C.: "Lithium decreases ClィイD1-ィエD1-ATPase activity and increases intracellular ClィイD1-ィエD1 concentration in cultured rat hippocampal neurons."Brain Res.. 821. 530-534 (1999)
  • Description: 「研究成果報告書概要(欧文)」より