1999 Fiscal Year Final Research Report Summary
Genetic operation of mouse embryos using exo-utero surgery
| Project/Area Number |
10680752
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Tokyo Metropolitan Organization for Medical Research (1999)
Tokyo Metropolitan Institute for Neuroscience (1998) |
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Principal Investigator |
KAWANO Hitoshi Tokyo Metropolitan Institute for Neuroscience, Staff scientist, 東京都神経科学総合研究所, 副参事 (20161341)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Kosei Nara Institute of Science and Technology, Assistant, 助手 (90206946)
KAWAMURA Koki Keio University, School of Medicine, Professor, 医学部, 教授 (40048286)
HAYASHI Shinji Tokyo Metropolitan Institute for Neuroscience, Staff scientist, 東京都神経科学総合研究所, 参事 (20076996)
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| Project Period (FY) |
1998 – 1999
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| Keywords | exo-utero surgery / mouse / development / antisense DNA / L1 / TAG-1 / proteoglycan / brain |
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| Research Abstract |
Recent development in the field of genetic and molecular technology has remarkably increased the knowledge on the mechanisms of the brain development. However, experimental approach to mammalian brain has been with much difficulty, because mammalian embryos develop in mother's uterus. To overcome this problem, we have developed the technique of the intrauteral surgery of mouse embryos.
Mice of 11 and 12 days of pregnancy was laparotomised and the adenovirus vector bearing Lac Z were demonstrated using x-gal staining. X-gal-positive cells were localized in the choroid plexus and a migrating rout from the rhombic lip to the cerebellar surface. These results indicate that the intrauteral surgery combined with adenovirus vector is useful tool to investigate the mechanisms underlying mammalian brain development.
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