2001 Fiscal Year Final Research Report Summary
Circadian gene expression of the circadian clock gene cluster kaiABC in cyanobacteria.
| Project/Area Number |
11304055
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
遺伝
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| Research Institution | Nagoya University, Center for Gene Research |
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Principal Investigator |
ISHIURA Masahiro Nagoya University, Center for Gene Research, Professor, 遺伝子実験施設, 教授 (20132730)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Takao Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10124223)
ITOH Shigeru Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (40108634)
ISHINO Yosizumi Biomolecular Engineering Research Institute, Principal investigator, 主任研究員
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| Project Period (FY) |
1999 – 2001
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| Keywords | Cyanobacteria / Circadian clock / clock gene / kaiABC / Gene expression / Autoregulation / Feedbak regulation / Cyanobacteria |
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| Research Abstract |
We analyzed the circadian clock in cyanobacteria, focused on the circadian clock gene cluster kaiABC and its gene products, KaiA, KaiB, and KaiC, and obtained the following results:
1 ) KaiC could bind to ATP and GTP, and it was autophosphorylated in the presence of ATP. These activities were necessary to clock oscillation.
2 ) We found SasA which bound to KaiC and enhanced the clock oscillation.
3 ) We analyzed the photolyase/cryptocrome family in cyanabacteria.
4 ) We found two KaiA-binding domains on KaiC.
5 ) We developed a promoter-trap method in cyanobacteria.
6 ) Pex functioned as a negative regulator for the expression of the kaiA gene.
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