2000 Fiscal Year Final Research Report Summary
Search of novel nuclear orphan receptors for fat-soluble ligands
| Project/Area Number |
11460035
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Institute of Molecular and Cellular Biosciences, The University of Tokyo |
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Principal Investigator |
KATO Shigeaki Institute of Molecular and Cellular Biosciences, The University of Tokyo Professor, 分子細胞生物学研究所, 教授 (60204468)
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| Co-Investigator(Kenkyū-buntansha) |
YANAGISAWA Junn Institute of Molecular and Cellular Biosciences, Assistant Professor, 分子細胞生物学研究所, 助手 (50301114)
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| Project Period (FY) |
1999 – 2000
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| Keywords | fat-soluble factor / vitamin D derivatives / non-steroid hormone / heterodimer / two-hybrid assay / VDR KO mice / gene promoter / double KO mice |
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| Research Abstract |
Some fat-soluble factors in intact aminals and diet act as ligands for nuclear receptors. In the present study, tissue-specific actions of vitamin D have been sudied by VDR KO mice, which develope rickets only after weaning. Furtherrmore, those mice were crossed with RXRγ Ko mice to establish double Ko mice for VDR and RXRγ, and the mice exhibited more over phenotypes, indicating a cross-talk between vitamin A and vitamin D.Conditional VDR KO in target tissues is under trial. We also searched novel nuclear receptors and found several candidates.
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[Publications] Watanabe, M., Yanagisawa, J., Kitagawa, H., Takeyama, K., Arao, Y., Suzawa, M., Kobayashi, Y., Ogawa, S., Yano, T., Yoshikawa, H., Masuhiro, Y., Kato, S.: "A subfamily of RNA binding DEAD-box proteins acts as an estrogen receptor α coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA"EMBO J.. 20, No.6. 1-12 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Li, M., Indra, A.K., Warot, X., Brocard, J., Messaddeq, N., Kato, S., Metzger, D., Chambon, P.: "Skin abnormalities generated by temporally-controlled RXRα mutations in adult mouse epidermis."Nature. 407. 633-636 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Kodera, Y., Takeyama, K., Murayama, A., Suzawa, M., Masuhiro, Y., Kato, S.: "Ligandtype specific interactions of peroxisome proliferator-activated receptor gamma with transcriptional coactivators."J.Biol.Chem.. 275. 33201-33204 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Kobayashi, Y., Kitamoto, T., Masuhiro, Y., Watanabe, M., Kase, T., Metzger, D., Yanagisawa, J., Kato, S.: "p300 Mediates functional synergism between AF-1 and AF-2 of estrogen receptor α and β by interacting directly with the N-terminal A/B domains."J.Biol., Chem.. 275. 15645-15651 (2000)
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「研究成果報告書概要(欧文)」より
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