2002 Fiscal Year Final Research Report Summary
Analysis of Thl/Th2 imbalance of systemic lupus erythematosus
| Project/Area Number |
11670458
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Juntendo University |
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Principal Investigator |
YAMADA Masato Juntendo University, School of Medicine, Dept. of Rheumatology, Assistant Professor, 医学部, 講師 (90311977)
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| Co-Investigator(Kenkyū-buntansha) |
TAMURA Naoto Juntendo University, School of Medicine, Dept. of Rheumatology, Assistant Professor, 医学部, 講師 (20227284)
FUKAZAWA Toru Juntendo University, School of Medicine, Dept. of Rheumatology, Assistant Professor, 医学部, 講師 (30301500)
YAGITA Hideo Juntendo University, School of Medicine, Dept. of Immunology, Associate Professor, 医学部, 助教授 (30182306)
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| Project Period (FY) |
1999 – 2002
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| Keywords | Systemic Lupus Erythemotosus / Lupus Nephritis / Th1 / Th2 / Chemokine Receptor / CCR5 / CCR4 / sjogren's syndrome |
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| Research Abstract |
Objective; Some chemokine receptors such as CCR5 and CCR4 are differentially expressed on Th1 and Th2 cells. To determine whether differential expression of the chemokine receptors was observed in patients with lupus nephritis, we examined the expression of CCR4 and CCR5 on peripheral blood lymphocytes and mononuclear cells infiltrated into renal tissue of patients with lupus nephritis
Methods; The expression of CCR4 and CCR5 on CD4^+ CD45RO^+ cells was analyzed by flow cytometry and compared between patients with systemic lupus erythematosus (SLE) and healthy controls. Correlation between the absolute number of CCR4^+ or CCR5^+ cells and clinical parameters was also analyzed. Mononuclear infiltrates in the renal tissue of SLE patients were analyzed for the expression of CCR4, CCR5, and CD4 by immunohistochemical staining.
Results; The absolute number of CCR4^+, but not CCR5^+ , T lymphocytes in the peripheral blood (PB) was significantly decreased in the patients with SLE compared to that in healthy controls and positively correlated with the serum levels of 3rd component of complement (C3) and 50% hemolytic unit of complement (CH50). Most of the CD4^+ T lymphocytes that infiltrated into the renal tissue of the patients with lupus nephritis expressed CCR4 but not CCR5
Conclusion; These results suggested that CCR4^+ T lymphocytes in PB, which represent Th2 cells, preferentially migrated into the renal tissue of the patients with lupus nephritis. The maldistribution of CCR4^+ T lymphocytes might be involved in the pathogenesis of lupus nephritis
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[Publications] Suzuki,N, Nakajima,A, Yoshino,S, Matsushima,K, Yagita,H, Okumura,K: "Selective accumulation of CCR5^+ T lymphocytes into inflamed joints of rheumatoid arthritis."Int Immunol. 11. 553-559 (1999)
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