1999 Fiscal Year Annual Research Report

慢性活動性EBウイルス感染症とEBウイルス関連リンパ増殖症におけるT細胞の解析

Research Project

Project/Area Number	11670766
Research Institution	Kyushu University
Principal Investigator	大賀正一九州大学, 医学部, 助手 (60233053)
Co-Investigator(Kenkyū-buntansha)	野村明彦九州大学, 医学部, 医員
Keywords	慢性活動性EBV感染症 / EBVウイルス量 / リンパ増殖性疾患 / Th1 / Th2 / 細胞内サイトカイン
Research Abstract	活性化T細胞とEBウイルス(EBV)のクローナル増殖を証明した慢性活動性EBV感染症(CAEBV)患児(J Pediatr Hematol/Oncol 20:342-346,1998&21:42-46,1999)を含む4名の末梢血より単核球を分離しcDNAを作成して、T細胞抗原受容体(TCR)Vβ領域遺伝子を用いたinverse PCR法によりTCRレパートアを解析した。症例ごとに異なる特定のVβを使用するクローンが増加しており、これはJβの使用状況からoligoclonal expansionであることが示された(Am J Hematol 61:26-33,1999)。つぎに患児の末梢血単核球を3時間培養しPMAとCaイオノフォアにて刺激してIFNγ/IL-4蛋白の細胞内発現(細胞内サイトカイン)をFACSにて解析した。伝染性単核症(IM)患者と比較して有意にTh0、Th1、Tc1細胞の割合が増加しIFNγ産生細胞の優位が示唆された。この現象をEBV感染T/NK細胞が担っているのか、感染細胞に対する反応性変化であるのかを検討するため、Cell Sorterにより分離したHLA-DR陽性および陰性T細胞のEBV-DNA量と細胞内サイトカインmRNAをReal time PCRを用いて定量し、IMの同細胞亜群と比較して検討中である。現在のところ、CAEBVではいずれも活性化T細胞にEBV感染量が多いことが明らかとなり二年次はこの解析を継続する。なおこのEBV定量法の妥当性を臍帯血移植後にEBV関連リンパ増殖症(PLD)患児の末梢血のモニターなどから確認し報告した(BMT 2000 in press)。

Research Products

(10 results)

All Other

All Publications (10 results)

[Publications] Ohshima K,Suzumiya J,Sugihara M,Nagafuchi S,Ohga S,et al.: "CD95(Fas) ligand expression of Epstein-Barr virus(EBV)-infected lymphocytes : a possible mechanism of immune evasion in chronic active EBV infection"Pathol International. 49(1). 9-13 (1999)
[Publications] Ohga S,Kimura N,Takada H,et al.: "Restricted diversification of T-cells in chronic active Epstein-Barr virus infection : Potential inclination to T-lymphoproliferative disease"Am J Hematol. 61(1). 26-33 (1999)
[Publications] Ohga S,Okada K,Ueda K,et al.: "Cerebrospinal fluid cytokine levels and dexamethasone therapy in bacterial meningitis"J Infect. 39(1). 55-60 (1999)
[Publications] Takada H,Ohga S,Mizuno Y,et al.: "Oversecretion of interleukin 18 in hemophagocytic lymphohistiocytosis : a novel marker of disease activity"Br J Hematol. 106(1). 182-189 (1999)
[Publications] Mizuno Y,Takada H,Uragami K,Ihara K,Kira R,Ryutaro Kira,Suminoe A,Ohga S,et al.: "Neurotropin-3 levels in cerebrospinal fluid from children with bacterial meningitis,viral meningitis, or encephalitis"J Child Neurol. 15(1). 19-21 (2000)
[Publications] Kajiwara M,Nonoyama S,Eguchi M,Morio T,Imai K,Okawa H,Kaneko M,Sako M,Ohga S,et al.: "WASP is involved in proliferation and differentiation of human haematopoietic"Br J Haematol. 107(2). 254-262 (1999)
[Publications] Sakai Y,Nakayama H,Matsuzaki A,Nagatoshi Y,Suminoe A,Honda K,Inamitsu T,Ohga S,Hara T: "Trisomy 10 in a child with acute nonlymphocytic leukemia followed by relapse with a different clone"Cancer Genet Cytogenet. 115(1). 47-51 (1999)
[Publications] Ohga S,Kanaya Y,Maki H,et al.: "Epstein-Barr virus-associated lymphoproliferative disease after a cord blood transplant for Diamond-Blackfan anemia"Bone Marrow Transplant. 25(2). 209-212 (2000)
[Publications] Ohga S,Takada H,Honda K,et al.: "Central nervous system T-lymphoproliferative disorder in chronic active Epstein-Barr virus infection"J Pediatr Hematol/Oncol. 21(1). 42-46 (1999)
[Publications] Nagatomo T,Ohga S,Saito M,et al.: "Streptococcus intermedius-brain abscess in chronic granulomatous disease"Eur J Pediatr. 158(10). 872-873 (1999)

1999 Fiscal Year Annual Research Report

慢性活動性EBウイルス感染症とEBウイルス関連リンパ増殖症におけるT細胞の解析

Principal Investigator

大賀 正一 九州大学, 医学部, 助手 (60233053)

Research Products

[Publications] Ohshima K,Suzumiya J,Sugihara M,Nagafuchi S,Ohga S,et al.: "CD95(Fas) ligand expression of Epstein-Barr virus(EBV)-infected lymphocytes : a possible mechanism of immune evasion in chronic active EBV infection"Pathol International. 49(1). 9-13 (1999)

[Publications] Ohga S,Kimura N,Takada H,et al.: "Restricted diversification of T-cells in chronic active Epstein-Barr virus infection : Potential inclination to T-lymphoproliferative disease"Am J Hematol. 61(1). 26-33 (1999)

[Publications] Ohga S,Okada K,Ueda K,et al.: "Cerebrospinal fluid cytokine levels and dexamethasone therapy in bacterial meningitis"J Infect. 39(1). 55-60 (1999)

[Publications] Takada H,Ohga S,Mizuno Y,et al.: "Oversecretion of interleukin 18 in hemophagocytic lymphohistiocytosis : a novel marker of disease activity"Br J Hematol. 106(1). 182-189 (1999)

[Publications] Mizuno Y,Takada H,Uragami K,Ihara K,Kira R,Ryutaro Kira,Suminoe A,Ohga S,et al.: "Neurotropin-3 levels in cerebrospinal fluid from children with bacterial meningitis,viral meningitis, or encephalitis"J Child Neurol. 15(1). 19-21 (2000)

[Publications] Kajiwara M,Nonoyama S,Eguchi M,Morio T,Imai K,Okawa H,Kaneko M,Sako M,Ohga S,et al.: "WASP is involved in proliferation and differentiation of human haematopoietic"Br J Haematol. 107(2). 254-262 (1999)

[Publications] Sakai Y,Nakayama H,Matsuzaki A,Nagatoshi Y,Suminoe A,Honda K,Inamitsu T,Ohga S,Hara T: "Trisomy 10 in a child with acute nonlymphocytic leukemia followed by relapse with a different clone"Cancer Genet Cytogenet. 115(1). 47-51 (1999)

[Publications] Ohga S,Kanaya Y,Maki H,et al.: "Epstein-Barr virus-associated lymphoproliferative disease after a cord blood transplant for Diamond-Blackfan anemia"Bone Marrow Transplant. 25(2). 209-212 (2000)

[Publications] Ohga S,Takada H,Honda K,et al.: "Central nervous system T-lymphoproliferative disorder in chronic active Epstein-Barr virus infection"J Pediatr Hematol/Oncol. 21(1). 42-46 (1999)

[Publications] Nagatomo T,Ohga S,Saito M,et al.: "Streptococcus intermedius-brain abscess in chronic granulomatous disease"Eur J Pediatr. 158(10). 872-873 (1999)

大賀正一九州大学, 医学部, 助手 (60233053)