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2000 Fiscal Year Final Research Report Summary

Analysis of the role of the expression of MSH receptor subspecies in growth, differentiation, and metastasis in pigment cells

Research Project

Project/Area Number 11670831
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKobe University

Principal Investigator

FUNASAKA Yoko  Kobe University School of Medicine, Department of Dermatology, Lecturer, 医学部・附属病院, 講師 (30209150)

Project Period (FY) 1999 – 2000
KeywordsMSH / MSH receptor / MC1R / melanin / PKC / PKA / tyrosinase
Research Abstract

1. MC1-R expression in keratinocytes : MC1-R was expressed in not only melanocytes but also keratinocytes and their expression was correlated with their differentiation state.
2. Correlation of MC1-R expression and metastatic ability in melanomas : Using the hybridoma of melanoma cells and macrophage, higher expression of MC1-R was proved to be correlated with metastatic ability.
3. Downregulation of the expression of tyrosinase related protein 2 (TRP2) by UVB induced apoptotic change of melanoma cells.
4. Expression of MC-R subfamily in human melanocytes/melanoma cells : In melanoma cells, all MC-R subfamilies (MC1-R, 2-R, 3-R, 5-R) except MC4-R was detected. In case of normal human melanocytes, MC1-R and 3-R was detected in all four strains of melanocytes, 5-R in two strains, 2-R and 4-R in one strain. This indicates that MC-R subfamily is differently expressed among different melanocytes, in contrast there is no heterogeneity in melanomas.
5. The role of PKA and PKC in the activation of tyrosinase : The antisense of PKCα and β, and inhibitor of PKC enhanced MSH-induced tyrosinase activity. The addition of dbcAMP increased intracellular level of cAMP and tyrosinase activity, but inhibitior of PKC suppressed tyrosinase activity. Taken together, PKC acts as an inhibitor of MSH-induced activation of tyrosinase but as an activator of cAMP-induced activation of tyrosinase.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Chakraborty AK: "Enhanced expression of melanocortin-1(MC1-R) in normal human keratinocytes during differentiation : evidence for increased expression of POMC peptides near suprabasal layer of epidermis."J Invest Dermatol. 112. 853-856 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Funasaka Y: "Expression of corticotropin Releasing Hormone in Malignant Melanoma"Ann NY Acad Sci. 885. 391-393 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chakraborty AK: "UV light and MSH receptors."Ann NY Acad Sci. 885. 100-117 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Funasaka Y: "Expression of Propiomelanocortin, corticotropin releasing hormone (CRH), and CRH receptor in malignant melanoma cells, nevus cells and normal human melanocytes."J Invest Dermatol (Symp Proc). 4. 105-110 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chakraborty AK: "Macrophage fusion upregulates mRNA for melanocortin 1 receptor buy not for the melonogenic proteins in Cloudman Sal melanoma cells."Pigment Cell Res. 12. 355-366 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Funasaka Y: "The effect of thioredoxin on the expression of proopiomelanocortin derived peptides and their melanocortin 1 receptor and cell Survival in normal human keratinocytes"J Invest Dermatol (Symp Proc). (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chakraborty AK, Funasaka Y, Pawelek JM, Nagahama M, Ito A, and Ichihashi M.: "Enhanced expression of melanocortin-1 receptor (MC1-R) in normal human keratinocytes during differentiation : evidence for increased expression of POMC peptides near suprabasal layer of epidermis"J Invest Dermatol. 112. 853-860 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Eunasaka Y, Sato H, and Ichihashi M.: "Expression of Corticotropin Releasing Hormone in Malignant Melanoma"Ann NY Acad Sci. 885. 391-393 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chakraborty AK, Funasaka Y, Slominski A, Bolognia J, Sodi S, Ichihashi M, and Pawelek JM.: "UV light and MSH receptors"Ann NY Acad Sci. 885. 100-117 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funasaka Y, Sato H, Chakraborty AK, Ohashi A, Churousos PG, and Ichihashi M: "Expression of proopiomelanocortin, corticotropin releasing hormone (CRH), and CRH receptor in malignant melanoma cells, nevus cells, and normal human melanocytes."J Invest Dermatol (Symp Proc). 4. 105-110 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chakraborty AK, Funasaka Y, Ichihashi M, Sodi S, and Pawelek JM: "Macrophage fusion upregulates mRNA for melanocortin 1 receptor but not for the melanogenic proteins in Cloudman S91 melanoma cells."Pigment Cell Res. 12. 355-366 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Funasaka Y, Chakraborty AK, Yodoi J, and Ichihashi M: "The effect of thioredoxin on the expression of proopiomelanocortin derived peptides and their melanocortin 1 receptor and cell survival in normal human keratinocytes"J Invest Dermatol (Symp Proc). (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2002-03-26  

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