2000 Fiscal Year Final Research Report Summary
Gene transfection in human keratinocyte and melanocyte
| Project/Area Number |
11671780
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
YAMAMOTO Yuhei Hokkaido Univ., Medical Hospital, Assistant Prof., 医学部・附属病院, 助手 (70271674)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIHARA Tsuneki Hokkaido Univ., Grad.School of Med., Chief Prof., 大学院・医学研究科, 教授 (20002157)
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| Project Period (FY) |
1999 – 2000
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| Keywords | transfection / ha-tagged vector / transfectant / lipofection / electroporation / gelsolin / gelsolin truncate / human primary melanoma |
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| Research Abstract |
1 At first, we studied the transfection of ha-tagged human gelsolin cDNA in human squamous carcinomacell line HEC46 and human melanoma cell line in stead of keratinocyte and melanocyte. We used human melanoma tissue and normal skin tissue as a control, and found out a unique 85Kda protein in Western blotting.
2 By Western blotting analysis of 38 melanoma samples for the expression of gelsolin using anti-gelsolin antibodies, we identified a new truncated variant of gelsolin p85 (GSNp85) lacking the C-terminal domain, which was always co-expressed with wild type gelsolin. The GSNp85 variant was more frequently expressed in vertical growth phase (VGP) melanomas as compared to radial growth phase (RGP) melanomas and was not expressed in other skin cancers, normal skin tissues, and nevi tissues except for 2 congenital junctional types. These data suggest that the occurrence of GSNp85 in melanoma may be a new indicator for the advanced stage of the tumor.
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