2000 Fiscal Year Final Research Report Summary
Introduction of tyrosine hydroxylase gene into the cells from brain for the treatment of parkinson's disease.
| Project/Area Number |
11672264
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
応用薬理学・医療系薬学
|
|---|
| Research Institution | KYUSHU UNIVERSITY |
|---|
Principal Investigator |
KOYABU Noriko Graduate Sch.of Pharm.Sci., KYUSHU UNIVERSITY Research Associate, 薬学研究院, 助手 (00315102)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIWADA Hiroshi Tokushima Univ., Faculty of Pharmaceutical Sciences, Prof., 薬学部, 教授 (50120184)
OHTANI Hisakazu Graduate Sch.of Pharm.Sci., KYUSHU UNIVERSITY Ass.Prof., 薬学研究院, 助教授 (70262029)
SAWADA Yasufumni Graduate Sch.of Pharm.Sci., KYUSHU UNIVERSITY Prof., 薬学研究院, 教授 (80114502)
KIKUCHI Hiroshi Daiichi Pharmaceutical Co., Ltd., 創剤研究所, 主任研究員
HARASHIMA Hideyoshi Hokkaido Univ., Faculty of Pharmaceutical Sciences, Prof., 薬学研究科, 教授 (00183567)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Keywords | Gene Therapy / HVJ-liposomes / Parkinson' disease / Brain capillary endothelial cells / Blood-brain barrier / Glial-Derived Neurotrophic Factor |
|---|
| Research Abstract |
1. The hemagglutation virus of Japan (HVJ)-liposomes were found to be a useful tool for the efficient introduction of macromolecules, including oligodeoxynucleotide, into cultured brain capillary endothelial cells in vitro.
2. Using HVJ-liposomes encapsulating a β-galactosidase gene expression vector, the gene was demonstrated to be efficiently transfered into cultured brain capillary endothelial cells in vitro.
3. The efficient transfer of gene into brain capillary in vivo was achieved by the administration of the HVJ-liposomes encapsulating a β-galactosidase gene expression vector into carotid artery. The expression of β-galactosidase at brain capillaries was found throughout the parenchyma.
4. The above results (2 and 3) indicate that the HVJ-liposome is a useful vector to transfer foreign gene into the brain capillary endothelium in vitro and in vivo.
5. Additional study demonstrated that the cultured mouse brain endothelial cells transfected with mouse glial-derived neurotrophic factor (GDNF) cDNA was found to secrete mature GDNF predominantly to abluminal side (brain side).
|
