2002 Fiscal Year Final Research Report Summary
Dynamic analysis of cellulose synthases in xylem cells
| Project/Area Number |
12306009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林産学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
ITOH Takao Wood Research Institute, Professor, 木質科学研究所, 教授 (70027168)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Kei'ichi Wood Research Institute, Lecturer, 木質科学研究所, 助手 (20238223)
SUGIYAMA Junji Wood Research Institute, Associate Professor, 木質科学研究所, 助教授 (40183842)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Cellulose synthases / GFP / GhCesA::GFP proteins |
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| Research Abstract |
(1) It was demonstrated that part of cellulose synthase, c-di-GMP binding domain, is localized in the specific particle rows in cell membrane.
(2) GhCesA::GFP was prepared for the visualization of TC movement
(3) Three different techniques that is microinjection, electro-polation, and particle-gun was examined to introduce GhCesA::GFP proteins into tobacco BY2 cells. The particle-gun method was the best to introduce the proteins in the cells with a little damages.
(4) The network structure was confirmed as endoplasmic reticulum (ER) by comparing the fluorescence pattern of GFP-fused proteins and DiOC6 staining images specific to ER.
(5) It was shown that the partial CesA3 gene can be expressed in ER.
(6) It was suggested that ChCesA::GFP proteins were localized in Golgi bodies as well by the distribution pattern of sphere-like fluorescence and their diameter similar to Golgi bodies.
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[Publications] Arioli T, Peng LC, Betzer AS, Burn J, Wittke W, Herth W, Camilleri C, Hofte H, Plazinski J, Birch R, Cork A, Glover J, Redmond J, Williamson RE: "Molecular analysis of cellulose biosynthesis in Arabidopsis"Science. 278. 717-720 (1998)
Description
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