2001 Fiscal Year Annual Research Report

唾液を用いた歯周病検査の開発

Research Project

Project/Area Number	12357014
Research Institution	HIROSHIMA UNIVERSITY
Principal Investigator	栗原英見広島大学, 歯学部, 教授 (40161765)
Co-Investigator(Kenkyū-buntansha)	伊藤公一日本大学, 歯学部, 教授 (90102607) 鴨居久一日本歯科大学, 歯学部, 教授 (10060419) 石川烈東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (10014151) 村山洋二岡山大学, 大学院・医歯学総合研究科, 教授 (50029972) 岩山幸雄朝日大学, 歯学部, 教授 (70028744)
Keywords	唾液 / 歯周病検査 / 外膜タンパク / ストレプトコッカスアンギノサス / 糖尿病 / マトリックスメタロプロテアーゼ-8 / 8-ハイドロキシデオキシグアニシ / 硫酸化グコサミノグルカン
Research Abstract	唾液を用いた歯周病検査の開発について多角的に検討した。侵襲性歯周災患者においてPorphyromonas gingivalis (P.g)に対するIgA抗体産生がみられ、唾液中の特異的IgA抗体測定によってP.g感染を検出できる可能性を示した。PCR(Polymerase chain reaction)法での唾液を使った歯周病原性細菌検査が可能であることを明らかにした。血清とActinobacillns actinomycetemcomitans (A.a)の外膜タンパク(Omp)が強く反応した歯周病患者の唾液を使って、A.aのOmpを抗原としたIgAレベルでのA.a感染の同定を行う有効な手段を示した。Streptococcus anginosus (S.a)をPCR法で特異的に検出する技術を確立し、上皮細織の抗菌ペプチド(hBD-2)とS.aの関連によって、S.aが病原性を示す際に必要な分子メカニズムの一端を解明した。歯周治療によりインスリン抵抗性や血糖コントロールを改善した糖尿病患者モデルを使うことで、唾液を用いた歯周病検査の開発に利用できることを明らかにした。糖尿病患者の唾液を検体とした生化学検査によって歯周疾患群と歯周疾患なし群とで有意差を認めず、糖尿病と歯周疾患の関連性を明らかにすることはできなかった。唾液中の好中球のエラスターゼ活性酸素産性能は歯周疾患の病態やリスク判定に有用である可能性を明らかにした。唾液中のMMP-8量の測定から、その活性型の値によって歯周疾患活動性の高い患者を特定できることを明らかにした。健常者に比べ歯周病患者では唾液中の酸化ストレス産物(8-OhdG)が有意に高く、歯周病診断の検査項目として有用なことを明らかにした。歯槽骨代謝マーカーとして有用性の示唆されている硫酸化グリコサミノグリカン(S-GAG)について、唾液での測定を行ったが、測定できなかった。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] Sakamoto, M. et al.: "Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA Libraries"Microbiol Immunol. 44. 643-652 (2000)
[Publications] Yano-Higuchi, K. et al.: "Prevalence of Bacteroides forsythus, Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival microflora of Japanese patients with adult and rapidly progressive periodontitis"J Clin Periodontol. 27. 597-602 (2000)
[Publications] Takeuchi, H. et al.: "New dental drug delivery system for removing mutans streptococci from the oral cavity : effect on oral microbial flora"Jpn J Infect Dis. 53. 211-212 (2000)
[Publications] Hanada, N. et al.: "Current Understanding of the Cause of Dental Caries"Jpn J Infect Dis. 53. 1-5 (2000)
[Publications] Suzuki, M. et al.: "Role of F_1F_0-ATPase in the growth of Streptococcus mutans GS5. 555-562"J Appl Microbiol. 88. 555-562 (2000)
[Publications] Sakamoto, M. et al.: "Rapid detection and Quantification of Five Periodontopathic Bacteria by Real-Time PCR"Microbiol Immunol. 45. 39-44 (2001)
[Publications] Takeuchi, Y. et al.: "Treponema socranskii, Treponema denticola, and Porphyromonas gingivalis are associated with severity of periodontal tissue destruction"Jpn J Infect Dis. 53. 211-212 (2000)
[Publications] Nomura, Y. et al.: "Mutans streptococci and Lactobacillus as risk factors for dental caries in 12-year-old children"Jpn J Infect Dis. 54. 43-45 (2001)
[Publications] Salam, MA. et al.: "Isolation of opportunistic pathogens in dental plaque, saliva and tonsil samples from elderly"Jpn J Infect Dis. 54. 193-195 (2001)
[Publications] Senpuku, H. et al.: "Inhibitory effects of MoAbs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of Streptococcus mutans"Microbiol Immunol. 54. 109-116 (2001)
[Publications] Muratat, T. et al.: "Periodontitis and serun interleukin-6 levels in the elderly"Jpn J Infect Dis. 54. 69-71 (2001)
[Publications] Takeuchi, H. et al.: "Clinical study of mutans streptococci using 3DS and monoclonal antibodies"Jpn J Infect Dis. 54. 34-36 (2001)
[Publications] Sobue, T. et al.: "Tissue inhibitor of metalloproteinases-1 (TIMP-1) and TIMP-2 directly stimulate the bone-resorbing activity of isolated mature osteoclasts"J Bone Miner Res. 16. 2205-2214 (2001)
[Publications] Shimizu, A. et al.: "Supportive Periodontal Treatment期にある歯周病患者の根面う蝕発症の実態"岡山歯学会雑誌. 20. 119-127 (2001)
[Publications] Tada, A. et al.: "The relation between tube feeding and Pseudomonas aeruginosa detection in the oral cavity"J Gerontol A Biol Sci Med Sci. 57. 71-72 (2002)
[Publications] Takane, M. et al.: "New biomaker evidence of oxidative DNA damage in whole saliva clinically health and periodontal diseased subjects"J Periodont. (in press). (2002)
[Publications] Komatsuzawa H. et al.: "Identification of six major outer membrane proteins from Actinobaullus actinomycetemcomitans"Gene. (in press).

2001 Fiscal Year Annual Research Report

唾液を用いた歯周病検査の開発

Principal Investigator

栗原 英見 広島大学, 歯学部, 教授 (40161765)

Research Products

[Publications] Sakamoto, M. et al.: "Comparison of the oral bacterial flora in saliva from a healthy subject and two periodontitis patients by sequence analysis of 16S rDNA Libraries"Microbiol Immunol. 44. 643-652 (2000)

[Publications] Yano-Higuchi, K. et al.: "Prevalence of Bacteroides forsythus, Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans in subgingival microflora of Japanese patients with adult and rapidly progressive periodontitis"J Clin Periodontol. 27. 597-602 (2000)

[Publications] Takeuchi, H. et al.: "New dental drug delivery system for removing mutans streptococci from the oral cavity : effect on oral microbial flora"Jpn J Infect Dis. 53. 211-212 (2000)

[Publications] Hanada, N. et al.: "Current Understanding of the Cause of Dental Caries"Jpn J Infect Dis. 53. 1-5 (2000)

[Publications] Suzuki, M. et al.: "Role of F_1F_0-ATPase in the growth of Streptococcus mutans GS5. 555-562"J Appl Microbiol. 88. 555-562 (2000)

[Publications] Sakamoto, M. et al.: "Rapid detection and Quantification of Five Periodontopathic Bacteria by Real-Time PCR"Microbiol Immunol. 45. 39-44 (2001)

[Publications] Takeuchi, Y. et al.: "Treponema socranskii, Treponema denticola, and Porphyromonas gingivalis are associated with severity of periodontal tissue destruction"Jpn J Infect Dis. 53. 211-212 (2000)

[Publications] Nomura, Y. et al.: "Mutans streptococci and Lactobacillus as risk factors for dental caries in 12-year-old children"Jpn J Infect Dis. 54. 43-45 (2001)

[Publications] Salam, MA. et al.: "Isolation of opportunistic pathogens in dental plaque, saliva and tonsil samples from elderly"Jpn J Infect Dis. 54. 193-195 (2001)

[Publications] Senpuku, H. et al.: "Inhibitory effects of MoAbs against a surface protein antigen in real-time adherence in vitro and recolonization in vivo of Streptococcus mutans"Microbiol Immunol. 54. 109-116 (2001)

[Publications] Muratat, T. et al.: "Periodontitis and serun interleukin-6 levels in the elderly"Jpn J Infect Dis. 54. 69-71 (2001)

[Publications] Takeuchi, H. et al.: "Clinical study of mutans streptococci using 3DS and monoclonal antibodies"Jpn J Infect Dis. 54. 34-36 (2001)

[Publications] Sobue, T. et al.: "Tissue inhibitor of metalloproteinases-1 (TIMP-1) and TIMP-2 directly stimulate the bone-resorbing activity of isolated mature osteoclasts"J Bone Miner Res. 16. 2205-2214 (2001)

[Publications] Shimizu, A. et al.: "Supportive Periodontal Treatment期にある歯周病患者の根面う蝕発症の実態"岡山歯学会雑誌. 20. 119-127 (2001)

[Publications] Tada, A. et al.: "The relation between tube feeding and Pseudomonas aeruginosa detection in the oral cavity"J Gerontol A Biol Sci Med Sci. 57. 71-72 (2002)

[Publications] Takane, M. et al.: "New biomaker evidence of oxidative DNA damage in whole saliva clinically health and periodontal diseased subjects"J Periodont. (in press). (2002)

[Publications] Komatsuzawa H. et al.: "Identification of six major outer membrane proteins from Actinobaullus actinomycetemcomitans"Gene. (in press).

栗原英見広島大学, 歯学部, 教授 (40161765)