2002 Fiscal Year Final Research Report Summary
Investigation of novel plant defense-related genes and the functional analysis
| Project/Area Number |
12460022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物保護
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| Research Institution | Nagoya University |
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Principal Investigator |
KAWAKITA Kazuhiro Nagoya University, Graduate School of Bioagricultural Sciences Associate Professor, 大学院・生命農学研究科, 助教授 (90186065)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIOKA Hirofumi Nagoya University, Graduate School of Bioagricultural Sciences Assistant Professor, 大学院・生命農学研究科, 助手 (30240245)
DOKE Noriyuki Nagoya University, Graduate School of Bioagricultural Sciences Professor, 大学院・生命農学研究科, 教授 (80023472)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Defence Response / Tabacco / Potato / Elicitor / Phytophthora infestans / Differential Display / Differential Hybridization / Subtraction |
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| Research Abstract |
The aim of this study is to screen novel resistance-related genes and analyze the function of these genes in plant defense responses against pathogen attack. We employed differential hybridization, subtraction and differential display methods to isolate elicitor-inducible genes of tobacco and potato using the plant-fungal elicitor from Phytophthora infestans system. Sequence analysis of cDNAs identified by differential hybridization in tobacco revealed similarities to genes for SAR8.2, glycine-rich protein, extensin, acyltransferase. Sequence analysis of cDNAs identified by subtraction in potato revealed similarities to genes for pathogenesis-related (PR) genes, glutathione-S-transferase, peroxidase, catalase, 1-aminocylopropane-1-carboxylic acid oxidase, and wound-inducible genes. Sequence analysis of cDNAs identified by differential hybridization in potato revealed similarities to genes for tyramine hydroxycinnamoyl transferase, glucose 6-phosphate dehydrogenase, pyruvate decarboxylse, and defense related-genes. We analyzed the function of two novel genes isolated by differential display in tobacco. One is elicitor-inducible cytochrome P450 gene designated CYP82E1. There is the possibility that CYP82E1 act in biosynthesis of barrier or antifungal compounds to limit invasion of pathogens. The other gene codes for a leucine-rich repeat (LRR) protein closely related to Cf genes in tomato and designated EILP (Elicitor-Inducible LRR Protein) gene. In transgenic tobacco plants overexpressing EILP, expression of defense-related genes against various elicitors increased and resistance against pathogenic bacteria was activated, implying that the product of EILP acts as a receptor-like protein amplifying signals in plants infected with pathogen and elicit defense responses.
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