2002 Fiscal Year Final Research Report Summary
Isolation of novel thermophilic organisms from marine hydrothermal environments and the development of new catalytic functions
| Project/Area Number |
12460093
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SAKO Yoshihiko Kyoto University Applied Biosciences Associate Professor, 農学研究科, 助教授 (60153970)
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| Co-Investigator(Kenkyū-buntansha) |
ISHINO Yoshizumi Kyusyu University Applied Biosciences Professor, 農学研究院, 教授 (30346837)
NOMURA Norimiti Kyoto University Applied Biosciences Instructor, 農学研究科, 助手 (10314246)
UCHIDA Aritsune Kyoto University Applied Biosciences Professor, 農学研究科, 教授 (50027190)
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| Project Period (FY) |
2000 – 2002
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| Keywords | hyperthermophilic microorganism / marine hydrothermal vent / Archaea / heat stable enzymes / homing endonuclease / hydrogen bacteria / Intron / restriction enzyme |
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| Research Abstract |
The purposes of this study are the isolation of useful new hyperthermophilic microorganisms for biotechnology from marine hydrothermal environments and the development of super catalytic functions of these thermophilic genes.
(1) Ten strains of aerobic hyperthermophilic archaeon Aeropyrum pernix were isolated from the submarine hydrothermal vents in Tachibana Bay, Nagasaki Prefecture, characterized the physiological properties and intron diversity. A new hyperthermophilic archaeon Pyrobaculum oguniense was isolated from Tuetate hotspring in Kumamoto Prefecture, which could grow most under aerobic condition over 90C although the isolate was also able to grow under microaerobic and anaerobic conditions. From the hydrothermal field at the Suiyo Seamount in the Izu-Bonin Arc, a new aerobic thermophilic bacterium Marinithermus hydrothermalis (opt. temp. 70C) and a new autotrophic hydrogen bacterium Persephonella hydrobenophila (opt. temp. 70C) were isolated and characterized.
(2) The 16S and 23S rDNA introns of A. pernix carried homing endonuclease genes which were expressed and characterized the properties. Both homing endonucleases showed high optimum temperature (90C) and recognized the specific long sequences.
(3) In hyperthermophilic archaeon A. pernix K1 a restriction enzyme activity (ApeK1) was found in crude extract. ApeK1 recognizing new split site was purified and characterized the properties. Molecular weight of ApeK1 was 40,000. The optimum temperature was 90C. The function unknown ORF558 of A. pernixK1 was identified the gene of ApeK1 from the result of amino acid sequence analysis.
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[Publications] Nomura, N., Morinaga, Y., Kogishi, T., Kim, E., Sako, Y., Uchida, A.: "Heterogenous yet similar introns reside in identical positions of the rRNA genes in natural isolates of the archaeon Aeropyrum pernix"Gene. 295・1-2. 43-50 (2002)
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「研究成果報告書概要(和文)」より
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[Publications] Takai, K., Inagaki, F., Nakagawa, S., Hirayama, H., Nunoura, T., Sako, Y., et al.: "Isolation and phylogenetic diversity of members of previously uncultured epsilon-Proteobacteria in deep-sea hydrothermal fields"FEMS Microbiology Letters. 218・1. 167-174 (2003)
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「研究成果報告書概要(和文)」より
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[Publications] Sako, Y., Nakagawa, S., Takai, K., Horikoshi, K.: "Marinithermus hydrothermalis gen. nov., sp. nov., a strictly aerobic, thermophilic bacterium from a deep-sea hydrothermal vent chimney"International Journal of Systematic and Evolutionary Microbiology. 53・1. 59-65 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nomura, N., Morinaga, Y., Kogishi, T., Kim, E., Sako, Y. and Uchida, A.: "Heterogenous yet similar introns reside in identical positions of the rRNA genes in natural isolates of the archaeon Aeropyrum pernix"Gene. 295. 43-50 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Takai, K., Inagaki, F., Nakagawa, S., Hirayama, H., Nunoura, T., Sako, Y., NealSon, K. H. and Horikoshi, K.: "Isolation and phylogenetic diversity of members of previously uncultured epsilon-Proteobacteria in deep-sea hydrothermal fields"FEMS Microbiology. 218. 167-174 (2003)
Description
「研究成果報告書概要(欧文)」より
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