2002 Fiscal Year Final Research Report Summary
MOLECULAR ANALYSIS OF ORIGIN OF JAPANESE DOMESTIC DOGS BY ANCIENT DNA
| Project/Area Number |
12460121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE |
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Principal Investigator |
ISHIGURO Naotaka OBIHIRO UNIVERSITY OF AGRICULTURE OF VETERINARY MEDICINE, LABORATORY OF VETERINARY PUBLIC HEALTH, ASSOCIATED PROFESSOR, 畜産学部, 助教授 (00109521)
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| Co-Investigator(Kenkyū-buntansha) |
TOMIOKA Naoto OKAYAMA UNIVERSITY OF SCIENCE, FACULTY OF SCIENCE, INSTRUCTOR, 理学部, 講師 (90241504)
HONGO Hitomi KYOTO UNIVERSITY, PRIMATE RESEARCH INSTITUTE, ASSISTANT PROFESSOR, 霊長類研究所, 助手 (20303919)
MATSUI Akira NARA NATIONAL CULTURAL PROPERTIES RESEARCH INSTITUTE, SENIOR RESEARCHER, 埋蔵文化財センター, 主任研究官 (20157225)
UEHARA Shizuka OKINAWA INTERNATIONAL UNIVERSITY, FACULTY OF ARTS, INSTRUCTOR, 文学部, 講師 (40320519)
EGAMI Miyuki OKINAWA INTERNATIONAL UNIVERSITY, FACULTY OF ARTS, ASSOCIATED PROFESSOR, 文学部, 助教授 (30320518)
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| Project Period (FY) |
2000 – 2002
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| Keywords | DNA / dog / mitochondria DNA / archaeology / ancient / haplotype / phylogeny / jomon |
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| Research Abstract |
Sequences of ancient mitochondrial DNA (mtDNA) from archaeological remains of Japanese dogs were determined from 145 ancient dog samples (mainly bones) from the Jomon, Yayoi, Kofun, Okhotsk and the kamakura Periods. Highly variable sequences of the mitochondrial control region (198bp) were amplified at least twice from independently prepared DNA extracts, and those from ancient samples were compared with * haplotypes from 178 modern dogs. The 198bp ancient mtDNA was amplified from 74 ancient dog samples, and the sequences were classified into 19 haplotypes comprising five modern haplotypes (M1, M2, M5, M10 and M11) identified in modern dogs. Three major clusters (CL1 to CL3) were distinguished within the modern dog control region using phylogenetic analysis; all ancient dogs belonged to the CL1 cluster. Origin of Hokkaido dogs was considered to be from southern dog population, but not from northern dog population. Ancient dogs in Okinawa Islands was derived from Eurasian continent through trade activity, and dogs in Gusuku Period had the same haplotypes to modern Ryukyu dog populations. The mtDNA from Japanese wolf was also determined, the genetical relationship among Japanese wolf, dogs and wolves were investigated. The phylogenetic analysis of mtDNA from Japanese wolf with those from dogs and wolves indicated that Japanese wolf genetically related to dogs and wolves from Eurasian continent.
To reinforce the canine mtDNA database, 600bp of mtDNA D-loop region was amplified from 365 dogs and determined for mtDNA sequence. The diversity of the 600bp sequences was classified into 64 haplotypes, including 46 newly discovered haplotypes, and the haplotypes were grouped into four clusters I to IV.
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