2001 Fiscal Year Final Research Report Summary
Development of DNA Detection Method using Photo-induced Electron Transfer
| Project/Area Number |
12555225
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
生物・生体工学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
IJIRO Kuniharu Hokkaido Univ., Research Institute for Electronic Science, Asso. Prof., 電子科学研究所, 助教授 (90221762)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Masaru Hokkaido Univ., Research Institute for Electronic Science, RA., 電子科学研究所, 助手 (00322850)
SHIMOMURA Masatsugu Hokkaido Univ., Research Institute for Electronic Science, Prof., 電子科学研究所, 教授 (10136525)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Photo-induced electron transfer / DNA / monolayer / nucleic acid base / fluorescence life time / gene / high sensitivity / non-label |
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| Research Abstract |
Double-stranded DNA is expected to show function as a molecular wire based on the π-electron array structure, so-called "π-ways", of stacked nucleic acid bases. DNA-mediated photo-induced electron transfer between electron donors and acceptors which are intercalated or bound to base pairs in polynucleotides has been investigated. We have reported that the amphiphilic intercalator, C_<18>AO^+, monolayer can be intercalated into DNA and other polynucleotides at the air-water interface and the composite monolayer with ordered DNA array can be transferred on the solid support.
Here we report, DNA-mediated photo-induced electron transfer in the monolayers containing electron donor and acceptor at the air-water interface. Binary mixed monolayers (an amphiphilic donor, C18AO+ and cationic amphiphile, 2C18N+2C1) and ternary mixture (an amphiphilic acceptor C12BphC5V2+) were prepared on the aqueous subphases of double-stranded DNA, RNA and other anionic polymers.
Pressure-area isotherms and fluorescence spectra were measured simultaneously at the air-water interface by Langmuir film balance equipping fluorescence microscope. Electron transfer quenching of C18AO+ fluorescence by C12BphC5V2+ molecule are clearly. Fluorescence quenching was enhanced by DNA and RNA, but not affected by synthetic anionic polymers. This phenomenon is observed only in the double-stranded DNA and RNA. Therefore hybridization of single-stranded DNA and RNA with complementary DNA and RNA can be detected by the photo-induced electron transfer through DNA and RNA combined with the ternary monolayer.
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Research Products
(12 results)
