2001 Fiscal Year Final Research Report Summary
Development of DNA vaccine therapy for allergic diseases in animals
| Project/Area Number |
12556056
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 展開研究 |
|---|
| Research Field |
Applied veterinary science
|
|---|
| Research Institution | THE UNIVERSITY OF TOKYO |
|---|
Principal Investigator |
TSUJIMOTO Hajime The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (60163804)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAKAGUCHI Masahiro National Institute of Infectious, Division of Immunology, A chief researcher, 免疫部, 主任研究官 (20170590)
MASUDA Kenichi The University of Tokyo, Graduate School of Agricultural and Life Sciences, assistant, 大学院・農学生命科学研究科, 助手 (40313077)
OHNO Koichi The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (90294660)
HIRAHARA Kazuki Sankyo Pharmaceutical, Third Institute of Biology, researcher, 第三生物研究所, 研究職
|
|---|
| Project Period (FY) |
2000 – 2001
|
| Keywords | Dog / DNA vaccine / Cry j1 / T-cell epitope / Atopic dermatitis |
|---|
| Research Abstract |
In order to establish an effective immunotherapy for allergic diseases using DNA vaccine, we examined the in vitro allergic reactions in spontaneously occurring allergic dogs and experimentally sensitized dogs and then explored in vivo efficacy of the immunotherapy using DNA vaccine.
Allergic reactions directed to Japanese cedar (Cryptomeria japonica, CJ) pollen antigens were examined in atopic dogs sensitive to CJ pollen. Most of the dogs were shown to have IgE directed to Cry j1, a major allergen of CJ pollen, and the IgE level was found to be high in the pollenation season of CJ. Next, T-cell epitopes of CJ pollen antigen were identified from the blastogenesis response to its overlapping peptides in these dogs. In an experimental immunotherapy trial using Cry j1 DNA vaccine in 4 atopic dogs, suppression of intradermal reaction and blastogenesis of peripheral blood lymphocytes was observed in parallel to the partial improvement of the clinical sings of atopic dermatitis.
On the other hand, CJ pollen-specific airway hypersensitivity could be induced in dogs experimentally sensitized with CJ pollen extract. After treatment of the dogs with Cry j1 DNA vaccine, threshold of the administered amount of CJ pollen extract in the intradermal reaction and airway provocation was shown to be increased in the treatment group, although the serum IgE level and lymphocyte blastogenesis response were not different between the treatment and control groups. Furthermore, the number of mast cells in the lung was smaller in the treatment group in comparison to the control group.
The present study provided evidence to indicate that DNA vaccine immunotherapy can be considered as an effective therapy for allergic diseases.
|
Research Products
(12 results)
-
-
-
-
-
-
-
[Publications] Masuda, K., Sakaguchi, M., Saiio, S., DeBoer, D.J., Fujiwara, S., Kurata, K., Yamashita, K., Hasegawa, A., Ohno, K., Tsujimoto, H.: "In vivo and in vitro tests showing sensitization to Japanese cedar (Cryptomeria japonica) pollen in atopic dogs."J.Vel.Med.Sci.. 62. 995-1000 (2000)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Masuda, K., Tsujimoto, H., Fujiwara, S., Kurata, K., Hasegawa, A., Taniguchi, Y., Yamashita, K., Yasueda, H., DeBoer, D.J., deWeck, A.L., Sakaguchi, M.: "IgE-crossreactivity to major Japanese cedar (Cryptomeria japonica) pollen allergens (Cry j 1 and Cry j 2) by ELISA in dogs with atopic dermatitis."Vet.Immuno.Immunopathol.. 74. 263-270 (2000)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Yamashita, K., Masuda, K., Sahaguchi, M., Odagiri, T., Nakao, Y, Yamaki, M., Hasegawa, A., Matsuo Y, DeBoer, D.J., Ohno, K., Tsujimoto, H.: "Experimental sensitization with Japanese cedar pollen in dogs."J.Vet.Med.Sci.. 62. 1223-1225 (2000)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Masuda, K., Kurata, K., Sakaguchi, M., Yamashita, K., Hasegawa, A., Ohno, K., Tsujimoto, H.: "Seasonal rhinitis in a cat sensitized to Japanese cedar (Cryptomeria japonica) pollen."J.Vet.Med.Sci.. 63. 79-81 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Sakaguchi, M., Masuda, K., Toda, M., Inouye, S., Yasueda, H., Taniguchi, Y., Nagoya, T., DeBoer, D.J., Tsujimoto, H.: "Analysis of canine IgE-binding epitope on the major allergen (Cry j 1) of Japanese cedar pollen with anti-Cry j1 monoclonal antibodies."Vet.Immunol.Immunopathol.. 78. 35-43 (2001)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Masuda, K., Sakaguchi, M., Saito, S., DeBoer, D.J., Yamashita, K., Hasegawa, A., Ohno, K., Tsujimoto, H.: "Seasonal atopic dermatitis in dogs sensitive to a major allergen of Japanese cedar (Cryptomeria japonica) pollen."Vet.Dermatol.. 13. 53-59 (2002)
Description
「研究成果報告書概要(欧文)」より
