2001 Fiscal Year Final Research Report Summary
Development of Pencil-type CCD Microscope for evaluating of renal glomerular microcirculation with normal and hypertensive Rat
| Project/Area Number |
12557065
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kawasaki College of Allied Health Professions |
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Principal Investigator |
TACHIBANA Hiroyuki Kawasaki College of Allied Health Professions, Department of Medical Engineering, Research Asscociates, 臨床工学科, 助手 (00241216)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAMATSU Osamu Kawasaki College of Allied Health Professions, Department of Medical Engineering, Lecture, 臨床工学科, 講師 (50208849)
MATSUMOTO Takeshi Kawasaki College of Allied Health Professions, Department of Medical Engineering, Associate Professor, 臨床工学科, 助教授 (30249560)
GOTO Masami Kawasaki College of Allied Health Professions, Department of Medical Engineering, Professor, 臨床工学科, 教授 (50148699)
KATAOKA Noriyuki Kawasaki College of Allied Health Professions, Department of Medical Engineering, Lecture, 臨床工学科, 講師 (20250681)
MOCHIZUKI Seiichi Kawasaki College of Allied Health Professions, Department of Medical Engineering, Associate Professor, 臨床工学科, 助教授 (60259596)
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| Project Period (FY) |
2000 – 2001
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| Keywords | CCD Microscope / glomerular microcirculation / Velocity mapping / 速度マッピング |
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| Research Abstract |
To evaluate hemodynamics of renal glomerular microcirculation, we developed a pencil-type CCD microscope for evaluating of renal glomerular microcirculation with normal and hypertensive Rat. The system consists of a pencil lens with light guides, a camera body, a control unit, light source, a monitor and a videocassette recorder. The tapered pencil lens (tip diameter : 1mm) contains relay lens surrounded by 8 annular optical-fiber light guides. The spatial resolution of this system is 0.86μm, and the temporal resolution is 16.7ms (60 frames/sec.). The focal depth is 20μm. The pencil lens probe was introduced into the superficial layer of kidney through the incision after the surgical procedure. Image of glomeruli and the adjacent afferent and efferent arterioles were continuously recorded on a videocassette tape. The time-sequential images of therenal zicrocirculation were digitized by a computer system. To measure erythrocyte velocities, a line segment was set along the glomerular capillaries in time-sequential digitized images and then a spatiotemporal image was constructed along that segment. The angle of striped pattern in the spatiotemporal image which reflects the erythrocyte velocity, was estimated to compute erythrocyte velocity vector mapping. We have succeeded in quantitatively evaluating the glomerular microcirculation by direct in-vivo observation with our intravital videomicroscope and image analyzing method. The spatiotemporal method is useful to evaluate the hemodynamic characteristic of the glomerular microcirculation in a rat, normal and abnormal.
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Research Products
(7 results)
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[Publications] S, Mochizuki, Y, Chiba, Y, Ogasawara, N, Kataoka, M, Goto, H, Tachibana, F, Kajiya: "Direct in situ evaluation of nitroglycerin-derived nitric oxide production in the canine and rat vascular walls at high temporal and spatial resolutions"Cardiovascular Engineering : An International Journal. 1-2. 85-91 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] D, Merkus, I, Vergrossen, 0, Hiramatsu, H, Tachibana, H, Nakamoto, E, Toyota, M, Goto, Y, Ogasawara, J, A, E, Spaan, F, Kajiya: "Stenosis differentially affects subendocardial and subepicardial arterioles in vivo."American Jouenal of Physiology. 280-4. H1676-H1682 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] T, Kajita, Y, Ogasawara, T, Matsumoto, H, Tachibana, H, Nakamoto, N, Kataoka, F. Shigeto, C, Tokuda, F, Kajiya: "Spatial correlation of mitochondorial function and flow evaluated with the resolution of microcirculatory flow control unit size in beating heart."Japanese Circulation Journal. 65. 473 (2001)
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「研究成果報告書概要(欧文)」より
