2001 Fiscal Year Final Research Report Summary
Genetic Structure of Hybrid Zones between Pinus pumila and P. pariflora var. pentaphylla.
| Project/Area Number |
12640679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
系統・分類
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| Research Institution | Chiba University (2001)
Kanazawa University (2000) |
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Principal Investigator |
WATANO Yasuyuki Chiba University, Faculty of Science Associate Professor, 理学部, 助教授 (70192820)
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| Co-Investigator(Kenkyū-buntansha) |
TANI Naoki Forestry and Forest Products Research Insutitute Department of Forest Genetics Researcher, 森林遺伝研究領域, 技官(研究職)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Pinus / introgressive hybridization / hybrid zone / chloroplast DNA / mitochondrial DNA / nuclear DNA / molecular hybrid index / microsatellite |
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| Research Abstract |
Pinus pumila is a creeping shrub which dominates in alpine zone. Pinus parviflora var. pentaphylla is a tall tree in sub-alpine to montane zones. Hybrid zones between the two species are observed mainly in mountains of Tohoku district, Japan. Pinus species exhibit a paternal cpDNA inheritance and a maternal mtDNA inheritance. Previous studies showed that paternal cpDNA flowed from P. parviflora to P. pumila, and in contrast maternal mtDNA from P. pumila to P. parviflora. In addition to diagnostic cpDNA and mtDNA markers, we developed multiple nuclear DNA markers in the present study. We carried out the screening of ESTP primers of Pinus taeda, and finally obtained ten diagnostic marker loci applicable to P. pumila and P. parviflora. Genetic structure of hybrid zones of Mt. Higasiazuma and Mts. Tanigawa were examined by molecular hybrid index. The hybrid index show the percentage of P. pumila marker alleles in the alleles examined. In Higasiazuma, the plants below the sub-alpine conifer forest composed of Abies and Tsuga (1800-1900 m) were genetically similar to P. parviflora, and the average of their hybrid index was 0.073. While, the hybrid index of the plants above the Abies-Tsuga forest were 0.949. This results suggest that the gene flow between the two species are effectively obstructed by the Abies-Tsuga forest. In Tanigawa lacking the sub-alpine conifer forest, hybrid index varied continuously from 0 to 1 according to the altitude of each individuals.
We also developed microsatellite markers applicable to P. pumila and P. parviflora. We concentrated DNA fragments having microsatellites by magnetic beads. We determined the nucleotide sequences of about 300 clones, and found 86 unique clones having microsatellites.
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Research Products
(6 results)
