2001 Fiscal Year Final Research Report Summary
STUDY OF REGULATORY REGION OF FISH TIMP GENE
| Project/Area Number |
12660185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fisheries chemistry
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
KINOSHITA Masato KYOTO UNIV., GRADUATE SCHOOL OF Agric., LECTURER, 農学研究科, 助手 (60263125)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Satoshi KOCHI UNIV. DEPARTMENT OF AGRIC. LECTURER, 農学部, 助手 (00314980)
TOYOHARA Haruhiko KYOTO UNIV., GRADUATE SCHOOL OF Agric., ASSOClATE PROF, 農学研究科, 助教授 (90183079)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Japanese flounder / proteinase / inhibitor / tenderization / meat |
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| Research Abstract |
The toughness is one of the most important elements, which define the commercial value of raw meat of fish. The degradation of extracellulr matrix is thought to be a cause of post-mortem tenderization of fish meat. Our previous study suggested that this tenderization is caused mainly by the metalloproteinases. Especially, matrix metalloproteinases (MMPs) are thought to be the most possible candidates for post-mortem tenderization of fish meat.
To elongate the toughness of fish meat during chilled storage, we proposed new technique mentioned below. There are endogenous proteinase inhibitors specific for MMPs. They are designated as tissue inhibitore of metalloproteinases (TIMPs). We attempt to elevate the quantity of TIMPs in fish meat during alive. At present, gene manipulated food has not get public acceptance yet So, we try to find suitable treatments which promote gene expression of TIMPs with the DNA sequence information of regulatory region of TIMP2b gene.
Using the BAC library of cloned Japanese flounder genomic DNA, we isolated two BAC clone containing TIMP2b gene. As a result of investigation of 5' flunking region of TIMP2b gene, we found a cis-element called CRE-BP1, which is known to response to stress such as UV, heat-shock, and osmotic pressure. To investigate the function of CRE-BP1, the luciferase expression vector which contains a series of deleted 5' flunking region of TIMP2b.
The 5' flunking region of TIMP2b responded to hyper-osmotic stress, but do not to hypo-osmotic, heat-shock, and UV stress. These results suggested that the possiblity to elongate the toughness of fish meat durng chilled storage.
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Research Products
(2 results)
