| Project/Area Number |
12670071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Wakayama Medical Universtiy |
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Principal Investigator |
MAEDA Masanobu Wakayama Med. Univ., Physiology, Professor, 医学部, 教授 (80181593)
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| Co-Investigator(Kenkyū-buntansha) |
OWADA-MAKABE Kyoko Wakayama Med. Univ., Physiology, Instructor, 医学部, 助手 (10203952)
TSUBOTA Yuji Wakayama Med. Univ., Physiology., Instructor, 医学部, 助手 (50197761)
YUKAWA Kazunori Wakayama Med. Univ., Physiology, Assist. Prof., 医学部, 講師 (20301434)
KUDO Hideaki UOEH, Anatomy, Assistant Professor, 医学部, 講師 (40289575)
DOI Yoshiaki UOEH, Anatomy, Associate Professor, 医学部, 助教授 (30258602)
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| Project Period (FY) |
2000 – 2001
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| Keywords | nucleus tractus solitarii / nitric oxide / nitric oxide synthase / green fluorescent protein (GFP) / fluorescence / brain / circulation / gene |
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| Research Abstract |
In order to investigate the motion of a single molecule of neuronal nitric oxide synthase (nNOS) in the nucleus tractus solitarius (NTS), we tried gene (green fluorescent protein and nNOS) transduction into the NTS.
1. The simple methods for gene transduction in vivo into the brain using plasmid vector have not been developed. We microinjected green fluorescent protein (GFP) Fusion TOPO Cloning Vectors with cationic lipids (LipofextAMINE 2000) into the NTS and the hypoglossal nucleus, and removed the brain three, five, seven and ten days after the microinjection, and observed the fluorescence.
2. We could observe the fluorescence four brains microinjected out of five rats five days after the injection of plasmid vectors. The intensity of the fluorescence was the strongest five days after the injection. It is considered that the gene expression was the strongest five days after the transduction.
3. Next, we microinjected LacZ Vectors with cationic lipids into the brain. However, we could not observe beta-gal. It may be considered that the efficiency of gene transduction into the brain using a little large vector may be low.
4. Several new transfection drugs have been developed rapidly. We tried some new transfection drugs. We could observe the beta-gal using a certain new transfection drug.
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