2001 Fiscal Year Final Research Report Summary
Functional analysis of Impact family imprinted genes : Multidimetional approaches with various model organisims
| Project/Area Number |
12670106
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General medical chemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
ITO Takashi Kanazawa Univ., Cancer Res. Inst., Professor, がん研究所, 教授 (90201326)
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| Project Period (FY) |
2000 – 2001
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| Keywords | GCN1 / GCN2 / YIH1 / autophagy |
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| Research Abstract |
The yeast Impact homolog YIH1 competes with the eIF2oc kinase GCN2 for GCN 1 -binding, because both share GI domain interacting with GCN1. The GI domain-mediated association of GCN2 with GCN1 is essential for the activation of eIF2a kinase activity of GCN2 and hence for stress response to amino acid starvation (general control response). We also found evidence for a crosstalk between general control reponse and the target of rapamycin (TOR) signaling pathways. Intriguingly, TOR and GCN2 have been shown to regulate autophagy and YIH1 was identified as a regulator of autophagy of nuclei. These results suggest that a molecular network contaning TOR, GCN2 and YIH 1 setms to regulate autophagy.
For mouse Impact, we started gene-targeting and one-hybrid screening for the factors interacting with the differentially methylted region in this gene. We also found HRH4/Hrh4 gene adjacent to IMPACT/Impact escape imprinting.
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Research Products
(13 results)
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[Publications] Ito, T., Chiba, T., Ozawa, R., Yoshida, M., Hattori, M., Sasaki: "A comprehensive two-hybrid analysis to explore the yeast protein interactome"Proc. Natl. Acad. Sci. USA. 98. 4569-4574 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., Kohda, D.: "Solution structure of the PX domain, a target of the SH3 domain"Nature Struct. Biol.. 8. 526-530 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Ago, T., Takeya, R., Kuribayashi, F., Hiroaki, H., Ito, T., et al.: "The PX domain as a novel phosphoinositide-binding module"Biochem. Biophys. Res. Commun.. 287. 733-738 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Mizushima, K., Miyamoto, Y., Tsukahara, F., Hirai, M., Sakaki, Y. & Ito, T: "A novel G-protein coupled receptor gene expressed in striatum"Genomics. 69. 314-321 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Okamura, K., Hagiwara-Takeuchi, Y., Li, T., Vu, T. H., Hirai, M., Hattori, M., Sakaki, Y., Hoffman, A. R. & Ito, T: "Comparative genome analysis of the mouse imprinted gene Impact and its nonimprinted human homolog IMPACT : toward the structural basis for species-specific imprinting"Genome Res.. 10. 1878-1889 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ito, T., Chiba, T., Ozawa, T., Yoshida, M., Hattori, M. & Sakaki, Y: "A comprehensive two-hybrid analysis to explore the yeast protein interactome"Proc. Natl. Acad. Sci. USA. 98. 4569-4574 (2001)
Description
「研究成果報告書概要(欧文)」より
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