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2001 Fiscal Year Final Research Report Summary

Functinal characterization of human mannose-binding lectin-associated serine protease (MASP) -1/3 and MASP-2

Research Project

Project/Area Number 12670119
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionFukushima Medical University School of Medicine

Principal Investigator

ENDO Yuichi  Fukushima Medical University School of medicine, Associate Professor, 医学部, 助教授 (20117427)

Project Period (FY) 2000 – 2001
Keywordscomplement / lectin pathway / MASP / CLs / Promoter / serine prtease
Research Abstract

In the lectin pathway, mannose-binding lectin (MBL)-associated serine proteases (MASPs) form complexes with polymeric lectin molecules which are involved in pattern recognition. Upon binding of the recognition molecules to carbohydrates on the surface of microorganisms, MASPs are converted to their active forms and initiate complement activation.
In this study, the 5'-flanking regions of the genes encoding human MASP-1 and MASP-2,Mkey enzymes in the lectin complement pathway, were isolated and characterized. The features of their promoters were compared with those of the human gene for Cls, an effector component of the classical pathway. The sequences upstream from the transcription start sites of the three genes contained the elements essential for transcription and liver-specific expression. Transient expression of constructs of these genes fused to the luciferase reporter gene confirmed their liver-specific expression and showed that the MASP promoters were slightly up-regulated by IL-1β. The stimulatory effects of IL-lβ on MASPl and 2 gene expression were abolished by the simultaneous presence of IL-6. MASPl promoter activity was also down-regulated by IFN-γ. In contrast, Cls promoter activity was strongly up-regulated by BL-6, IL-1β and IFN-1γ. These results suggest that EL-6 and IFN-γ may have a role in switching the host defense system from the lectin pathway to the classical pathway.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Sekine H.: "An ancient lectin-dependent complement system in an ascidian: novel lectin isolated from the plasma of the solitary ascidian"J.Immunol.. 167. 4504-4510 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Stover C.: "The human gene for mannan-binding lectin-associated serine protease-2 (MASP-2), the effector component of the lectin route"Genes Immun.. 2. 119-127 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kenjo A.: "Cloning and characterization of novel ficolins from the solitary ascidian, Halocynthia roretzi"J.Biol.Chem.. 276. 19959-19965 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ariga H.: "A correlation between Try290Cys in the FGFR2 gene and phenotype of Pfeiffer syndrome type 2"Pediatr.Int.. 43. 293-295 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakagawa M.: "Polymorphic expression of decay-accellerating factor in human colorectal cancer"J.Gastoenterol.Hepatol.. 16. 184-189 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watabe K.: "Structure, expression and chromosome mapping of MLZE, a novel gene which is preferentially expressed in"Jpn.J.Cancer Res.. 92. 140-151 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsushita M, Endo Y. Fujita T.: "Complement-activating complex of ficolin and mannose-binding lectin-associated serine protease"J Immunol. 164. 2281-2284 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yabuta N, Fujii T, Copeland N G, Gilbert D J, Jenkins N A, Nishiguchi H, Endo Y, Toji S, Tanaka H, Nishimune Y, Nojima H.: "Structure expression and chromosomal mapping of LATS2 a mammalian homologue of the Drosophila tumor suppressor gene lats/wart"Genomics. 63. 263-270 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shibuya A, Sawamoto N, Shimizu Y, Shibuya K, Osawa M, Hiroyama T, Eyre HJ, Sutherland GR, Endo Y, Fujita T, Miyabashi T, Sakano S, Tsuji T, Nakayama E, Phillips JH, Lanier LL, Nakauchi H.: "Fca/m receptor mediates endocytosis of TgM-coated microbes"Nature Immunology. 1. 441-446 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watabe K, Asada H, Endo Y. Kobayashi T, Kawamoto K, Itami S, Takao S, Shinomura Y, Aikou T, Yoshikawa K, Matsuzawa Y, Kitamura Y, Nojima H.: "Structure expression and chromosome mapping of MLZE a novel gene which is preferentially expressed in metastatic melanoma cells"Jpn J Cancer Res. 92. 140-151 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakagawa M, Mizuno M, Kawada M, Uesu T, Nasu J, Takeuchi K, Okada H, Endo Y, Fujita T, Tsuji T.: "Polymorphic expression of decay-accellerating factor in human colorectal cancer"J Gastoenterol Hepatol. 16. 184-189 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ariga H, Endo Y. Ujiie N, Fujita T, Ishii T, Ishibashi N. Suzuki H.: "A correlation between Try290Cys in the FGFR2 gene and phenotype of Pfeiffer syndrome type 2"Pediatr Int. 43. 293-295 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kei|jo A, Takahashi M, MatsusHita M, Endo Y. Nakata M, Mizuochi T, Fujita T.: "Cloning and characterization of novel ficolins from the solitary ascidian Halocynthia roretzi"J Biol Chem. 276. 19959-19965 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Stover C, Endo Y, Takahashi M, Lvnch N, Vorup-Jensen T, Thiel S, Jensenius J, Friedl H, Gregory S, Hankeln T, Fujita T, Schwaeble W.: "The human gene for mannan binding lectin-associated serine protease-2 (MASP-2) the effector componenet of the lectin route of complement activation is part of a tightly linked gene cluster on chromosome lp36.2-3."Genes Immun. 2. 119-127 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekine H, Kenjo A, Azumi K, Ohi G, Takahashi M, Kasukawa R, Ichihara N, Nakata M, Mizuochi T, Matsushita M, Endo Y, Fuiita T.: "An ancient lectin-dependent complement system in an ascidian novel lectin isolated from the plasm of the solitary ascidian Halocynthia roretzi"J Immunol. 167. 4504-4510 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsushita M, Endo Y. Hamasaki N, Fujita T.: "Activation of the lectin complement pathway by ficolins"Int Immunopharmacol. 1. 359-363 (2001)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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