• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2001 Fiscal Year Final Research Report Summary

Function of Protein Kinase PASK enriched in Transporting Epithelia

Research Project

Project/Area Number 12670133
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionMie University

Principal Investigator

USHIRO Hiroshi  Mie Univ., Faculty of Medicine, Associate Professor, 医学部, 助教授 (10151854)

Co-Investigator(Kenkyū-buntansha) TSUTSUMI Tomonari  Mie Univ., Faculty of Medicine, Assistant, 医学部, 助手 (40273370)
KAYAHARA Tatsuro  Mie Univ., Faculty of Medicine, Assistant Professor, 医学部, 講師 (20024705)
NAKANO Katsuma  Suzuka Univ. of Medical Science, Faculty of Health Science, Professor and Dean, 保健衛生学部, 教授 (30024623)
Project Period (FY) 2000 – 2001
KeywordsProtein kinase / Ste20-related kinase / Cytoskeleton / Actin-binding protein / Cellular stress / Distal convoluted tubule of kidney / Choroid plexus of brain / Gene targeting
Research Abstract

PASK is a Ste20-related protein kinase enriched in adrenal zona glomerulosa cells and transporting epithelia such as brain choroid plexus and gastric parietal cells. The molecular function and physiological roles of PASK have been investigated in this study, and we have obtained the following results :
1. PASK associates actin and tubulin in vivo, and in its kinase domain has F-actin-binding activity. In cultured cells, PASK translocates from the cytosol to cytoskeleton upon stimulation by cellular stresses like heat shock and hypersonic NaCl. These results suggest that PASK is involved in the regulation of cytoskeleton during responses to cellular stresses.
The C-terminal non-catalytic domain binds cytoskeleton when cells are exposed to celluar stresses, while the kinase domain binds cytoskeleton regardless exposure to the celluar stresses. These results suggest that PASK binds cytoskeleton through its kinase domain to actin cytoskeleton and through the C-terminal domain to cytoskeleton via non-actin protein(s).
2. PASK-deficient mice generated by targeted disruption of the PASK gene exhibit reduced fertility and shows anomalies in the reproductive organs : PASK deficient males have testes that were 77 % of the normal size and the uterine of mutant females is 2- to 3-fold larger than the wild-type. Portions of the seminiferous tubules of sterile mutant males contain no spermatids, while such tubules are rare in the fertile mutants. These results suggest that PASK is involved in the reproduction. The mechanisms by which PASK deletion affects the reproduction, including the cause of anomalies in the mutant reproductive organs, are currently under investigation in this laboratory.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Katsuma Nakano: "Neural circuits and topographic organization of the basal ganglia and related regions"Brain & Development. 22. 5-16 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomonari Tsutsumi: "Proline-and alanine-rich Ste20-related kinase associates with F-actin and translocates from the cytosol to cytoskeleton upon cellular stresses"The Journal of Biological Chemistry. 275. 9157-9162 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hong Lai: "Morphological evidence for a vestibulo-thalamo-striatal pathway via the parafascicular nucleus in the rat"Brain Research. 872. 208-214 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hisashi Onishi: "Surgical anatomy of the medial segment (S4) of the liver with special reference to bile ducts and vessels"Hepatogastroenterology. 47. 143-150 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuma Nakano: "Neural circuits and functional organization of the striatum"Journal of Neurology. 274. 1-15 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanemichi Chiba: "Efferent projections of infralimbic and prelimbic areas of the medial prefrontal cortex in the Japanese Monkey, Macaca fuscata"Brain Research. 888. 83-101 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野勝磨: "「視床-update」視床の構造 形態の面から"Clinical Neuroscience. 18. 18-21 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野勝磨: "大脳基底核の構造と機能 特集 Pasrkinson病治療の進歩 -神経内科と脳神経外科の接点をめぐって-"日本臨牀. 58. 2-6 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野勝磨: "大脳基底核の機能形態学 (特集 大脳基底核をめぐって)"Kinesis. 5. 10-13 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野勝磨: "大脳基底核の構造 (特集「Parkinson病をめぐって)"Clinical Neuroscience. 19. 14-17 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuma Nakano: "The Basal Ganglia VI (eds.A.M.Graybiel, M.R.DeLong)(分担)"Plenum, New York(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuma Nakano: "The Basal Ganglia Vll (eds.L.M.Faull, Louise F.B.Nicholson)(分担)"Plenum, New York(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野勝磨: "「視床」(板倉徹、前田敏博 編著) 視床の運動系神経回路(分担)"ブレーン出版、東京(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中野勝磨: "神経解剖学(J.H.Martin著 嶋井和世、出浦滋之監訳) (共訳) 第8、9章"広川書店、東京. 474 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Katsuma Nakano: "Neural circuits and topographic organization of the basal ganglia and related regions"Brain & Development. 22. 5-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomonari Tsutsumi et al.: "Proline-and alanine-rich Ste20-related kinase associates with F-actin and translocates from the cytosol to cytoskeleton upon cellular stresses"The Journal of Biological Chemistry. 275. 9157-9162 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hong Lai et al.: "Morphological evidence for a vestibulo-thalamostriatal pathway via the parafascicular nucleus in the rat"Brain Research. 872. 208-214 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hisashi Onishi et al.: "Surgical anatomy of the medial segment (S4) of the liver with special reference to bile ducts and vessels"Hepatogastroenterology. 47. 143-150 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuma Nakano et al.: "Neural circuits and functional organization of the striatum"Journal of Neurology. 274. 1-15 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanemichi Chiba et al.: "Efferent projections of infralimbic and prelimbic areas of the medial prefrontal cortex in the Japanese Monkey, Macaca fuscata"Brain Research. 888. 83-101 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuma Nakano et al.: "Superficial and deep thalamo-cortical projections from the oral part of the ventral lateral thalamic nucleus (VLo) receiving inputs to the internal pallidal segment (GPi) and cerebellar dentate nucleus in the macaque monkey"In : The Basal Ganglia VI, eds. A. M. Graybiel, M. R. DeLong, Plenum Press, New York (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsuma Nakano et al.: "Distribution of pontomesencephalic neurons projecting to the medullary reticular areas and spinal cord in relation to the pedunculopontine inucleus in the monkey"In : The Basal Ganglia VII, eds. L. M. Faull, and Louise F. B. Nicholson, Plenum Press, New York, (in press).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2003-09-17  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi