| Research Abstract |
1) We foundhistologic grade using MIB-1score was themost important prognostic factor in dedifferentiated liposarcomas of the retroperitoneum and synovial sarcomas.
2) We studied immunoreactivity for β-catenin, a cadherin undercoat protein, in 44 cases of synovial sarcoma. We found that nuclear expression of β-catenin, that was present in 57% of cases, was associated with an adverse outcome.
3) We examined for the presence of tumor-specific fusion gene transcripts by a reverse transcription polymelase chain reaction assay using RNA samples from the frozen tumor tissue of synovial sarcoma and adult neuroblastoma, and found molecular diagnosis was successfully performed by this analysis.
4) We examined the validity and reproducibility of diagnosis of histologic type and of 2 grading systems for adult soft-tissue sarcomas by comparing independent diagnoses of 4 pathologists and the diagnosis of the expert panel at National Cancer Center We obtained the results that diagnosis of the type of soft-tissue sarcoma for synovial sarcoma, small round-cell sarcoma, and liposarcoma and the MIB-1 grading system based on tumor differentiation were highly valid and reproducible among pathologists.
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