2001 Fiscal Year Final Research Report Summary
Analysis of viral gene products controlling latent and lytic replication of human herpesvirus 8
| Project/Area Number |
12670282
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
UEDA Keiji Osaka University, Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (00221797)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANISHI Koichi Osaka University, Graduate School of Medicine, Professor, 医学系研究科, 教授 (10029811)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Human Herpesvirus 8 / Kaposi's sarcoma-associated herpesvirus (KSHV) / Reactivation and transcription activator / ORF50(RTA) / latency / reactivation / γ-herpesvirus / OCT1 / K5 |
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| Research Abstract |
Human herpesvirus 8 (HHV8) or Kaposi's sarcoma-associated herpesvirus (KSHV) is a member of γ-herpesviridae and supposed to be a causative agent for Kapasi' s sarcoma (KS) and primary effusion lymphoma (PEL). We investigated the molecular mechanism of the onset of lytic replication. We identified lytic gene products, RTA, K5, K8, K9 etc. and generated monoclonal antibodies against such gene products. We also isolated TPA non-reactive cell clones infected with KSHV from the parental BCBL1. Using these, we clarified that RTA was a key transcriptional regulator for the reactivation, that RTA upregulated K9 gene through non-DNA binding mechanism, that RTA upregulated its own promoter through the interaction with OCT1 and hat K5 downregulated the MHC class I, which lead to the immune evasion during lytic replication.
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Research Products
(12 results)
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[Publications] Ueda.K, Ishikawa, K., Nishimura, K., Sakakibara, S., De, E., Yamanishi, K.: "Kaposi's sarcoma-associated herpesvirus (human herpesvirus8) replication and transcription factor activates the K9 (vIRF) gene through two distinct cis elements by a non-DNA binding mechanism"J. Virol.. (in press). (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nishimura, K., Ueda, K., Sakakibara, S., Ishikawa, K., Chen, J., Okuno, T., Yamanishi, K.: "Functional analysis of Kaposi's sarcoma-associated herpesvirus RTA in an RTA-depressed cell line."J. Hum. Virol.. 4. 296-305 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Chen, J., Ueda, K., Sakakibara, S., Okuno, T., Parravicinim, C., Corbellino, M., Yamanishi, K.: "Activation of latent Kaposi's sarcoma-associated herpesvirus by demethylat ion of the promoter of lytic transactivator"PNAS. 98. 4119-4124 (2001)
Description
「研究成果報告書概要(和文)」より
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[Publications] Ueda, K., Ishikawa, K., Nishimura, K., Sakakibara, S., De, E., Yamanishi, K.: "Kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) replication and transcription factor activates the K9 (vIRF) gene through two distinct cis elements by a non-DNA binding mechanism"J. Virol.. (in press). (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nishimura, K., Ueda, K., Sakakibara, S., Ishikawa, K., Chen, J., Okuno, T., Yamanishi, K.: "Functional analysis of Kaposi's sarcoma-associated herpesvirus RTA in a n RTA-depressed cell line"J. Hum. Virol.. 4. 296-305 (2001)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Chen, J., Ueda, K., Sakakibara, S., Okuno, T., Parravicinim, C., Corbellino, M., Yamanishi, K.: "Activation of latent kaposi's sarcoma-associated herpesvirus by demethylation of the promoter of lytic transactivator"PNAS. 98. 4119-4124 (2001)
Description
「研究成果報告書概要(欧文)」より
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