Complex effects of several chlorinated organic compounds mimicking hormone actions on rat offspring through different exposure routes

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12670321
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Kyushu University
• Principal Investigator: INOUE Naohide Faculty of Medical Sciences, Professor, 大学院・医学研究院, 教授 (00131904)
• Co-Investigator(Kenkyū-buntansha): HIRATA Miyuki Faculty of Medical Sciences, Assistant, 大学院・医学研究院, 助手 (30156674) ; TANAKA Akiyo Faculty of Medical Sciences, Assistant Professor, 大学院・医学研究院, 講師 (10136484) ; MAKITA Yuji Faculty of Medical Sciences, Associate Professor, 大学院・医学研究院, 助教授 (30209407) ; OMURA Minoru Faculty of Medical Sciences, Assistant, 大学院・医学研究院, 助手 (50243936)
• Project Period (FY): 2000 – 2002
• Keywords: Chlorinated compounds / P, p'-DDE / PDB (para-dichlorobenzene) / different exposure sources / Complex effects / Oral intake

Research Abstract

Effects of concurrent exposure to paradichlorobenzene (PDB) and p, p'-DDE (DDE) on rat offspring were investigated in pregnant Wistar rats by oral administration. Twenty-four pregnant Wister rats (dams) were divided into three groups; namely control group, PDB group and PDB+DDE group. Control group rats were fed with normal rat chow, CE-2, PDB group rats with CE-2 containing 25ppm (25μg/g CE-2) PDB and PDB+DDE group rats with CE-2 containing 25ppm PDB+125ppm DDE. Dams had been fed with each special chow for 42 days from gestational day 1 (GD1) to postpartum day 21 (PPD 21). Pregnant courses and delivery outcomes were evaluated subsequently. Rat offspring were culled on postnatal day 2 (PND 2) to four males and females each, in order to avoid differences of feeding states. Rat offspring had been fed with CE-2 after weaning. The growth and development of rat offspring were also observed continuously until 48 weeks of age. Autopsies were performed at 6, 16, 32, 42 weeks of age. Effects of concurrent exposure to PDB and DDE on reproductive organs, visceral organs and serum hormone levels were investigated in detail at autopsy. Perinatal exposure of these two substances produced neither anomaly of reproductive organs nor abnormal developments to offspring. Furthermore, no additive or synergistic effects of PDB and DDE were observed in this experiment.

Research Products

• [Publications] Makita Y, Matsuura T, Inoue N et al.: "Systemic toxicity of p,p'-DDE in aged male Wistar rats following oral administration"Fukuoka Acta Medica. 94(4)(in press). (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 槇田裕之, 田中昭代, 井上尚英他: "R,p-DDEとトリブチルスズの複合投与による影響"日本衛生学会誌. 56(1). 214-214 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Omura M, Makita Y, Inoue N et al.: "Onset of spermatogenesis is accelerated by gestational administration of 1,2,3,4,6,7-hexachlorinated naphthalene"Environmental Health Perspective. 108(6). 539-544 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yuji Makita, Toshiaki Matsuura, Rika Ogata, Minoru Omura, Akiyo Tanaka, Miyuki Hirata, Naohide Inoue: "Systemic toxicity of p,p'-DDE in aged male Wistar rats following oral administration"Fukuoka Acta Med. 94(4), in press. (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yuji Makita, Akiyo Tanaka, Miyuki Hirata, Minoru Omura, Rika Ogata, Naohide Inoue: "Effects of combined administration of p,p'-DDE and tributyltin (TBT)"Jpn J Hygiene. 56. 214-214 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Minoru Omura, Yoshito Masuda, Miyuki Hirata, Akiyo Tanaka, Yuji Makita, Rika Ogata, Naohide Inoue: "Onset of Spermatogenesis Is Accelerated by Gestational Administration of 1,2,3,4,6,7-Hexachlorinated Naphthalene"Environ Health Perspect. 108(6). 539-544 (2000)
  • Description: 「研究成果報告書概要(欧文)」より