2001 Fiscal Year Final Research Report Summary
Analysis of cell cycle regulation of hematopoietic cells derived from patients with myelodysplastic syndrome
| Project/Area Number |
12670793
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | HYOGO COLLEGE OF MEDICINE |
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Principal Investigator |
YAMAMOTO Masuji Hyogo College of Medicine,Department of Pediatrics,Associate Professor, 医学部, 助教授 (40200844)
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| Project Period (FY) |
2000 – 2001
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| Keywords | cell cycle / MDS (myedysplastic syndrome) / TGF-b |
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| Research Abstract |
The effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) and transforming growth factor-bl (TGF-bl) on cell cycle regulation of SKM-1 myelodysplastic syndrome (MDS) cells was analysed. Our results indicated that TGF-bl-mediated Gl arrest correlated with the downregulation of cdk4, cdk6 and cyclin D2, and that abrogation of TGF-bl-mediated Gl arrest by GM-CSF was caused by the constitutive overexpression of cyclin D2 and cdk6 but not cdk4.The relationship between the cell surface antigen and the cell cycle regulation of human bone marrow hematopoietic progenitor cells was further analysed. Our results suggested that the cell cycle arrest of human bone marrow hematopoietic progenitor cells by TGF-b was associated with stage-specific expression of c-kit and CD38.These phenomenon may shed light on the etiology of hatatopoietic disorder, such as MDS.
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Research Products
(7 results)
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[Publications] Hara, J., Park, Y-D., Yoshioka, A., Yumura-Yagi, K., Koudera, U., Hosoi, G., Sako, M.Kosaka, T., Sano, K., Misu, H.y Mabuchi, 0., Aoyagi, N., Yamamoto, M., Tawa/.A., Miyata/ H.-, Tanaka, H., Kikkawa, M., Shimodera, M.and Kawa-Ha, K.: "Intensification of chemotherapy using block therapies as consolidation and reinduction therapies for acute lymphoblastic leukemia during childhood."Int J Hematol. 74. 165-172 (2001)
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「研究成果報告書概要(欧文)」より
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[Publications] Kojima, S., Hibi, S.Kosaka, Y., Yamamoto, M., Tsuchida, M., Mugishima, H., Sugita, K., Yabe, H., Ohara, A.., and Tsukimoto, I.: "Immunosuppressive therapy using antithymocyte globulin, cyclosporine, and danazol with or without human granulocyte colony -stimulating factor in children with acquired aplastc anemia."Blood.. 96. 2049-2054 (2000)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nakazawa, T., Agematsu, K., Yasui, K., Onodera, T., Inoue, R., Kaneko, H., Kondo, N., Yamamoto, M., Kayagaki, N., Yagita, H., Okumura, K., Komiyama, A.: "Cytolytic mechanisms involved in non-MHC-restricted cytotoxicity in Chediak-Higashi syndrome."Clin Exp Immunol. 118. 108-114 (1999)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ohtsuki, F., Yamamoto, M., Nakagawa, T., Tanizawa, T., Wada, H.: "Granulocyte-macrophage colony -stimulating factor abrogates transforming growth factor-b1-mediated cell cycle arrest by up-regulating cyclin D2/cdk6"British J. Hematol.,. 98. 520-527 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Yamamoto, M., Nakagawa, M., Ichimura, N., Ohtsuki, F., Ohtsuka, Y., Tsujino, Y., Tanaka, A., Kamiya, T., Wada, H: "Lymphoblastic transformation of chronic myelomonocytic leukemia in an infant."Am. J. Hematol.,. 52. 212-214 (1996)
Description
「研究成果報告書概要(欧文)」より
