2002 Fiscal Year Final Research Report Summary
Study of the mechanism of tubuloglomerular feedback in macula densa
| Project/Area Number |
12671053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Saitama Medical School (2002)
Jikei University School of Medicine (2000-2001) |
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Principal Investigator |
HASEGAWA Hajime SAITAMA MEDICAL SCHOOL, ASSISTANT PROFESSOR, 医学部, 講師 (30231517)
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| Project Period (FY) |
2000 – 2002
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| Keywords | tubuloglomerular feedback / Na-K-2Cl cotransporter / aquaporin / renal failure / DM nephropathy |
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| Research Abstract |
In this study, the involvement of tubuloglomerular feedback (TGF) abnormality was studied. First, macula densa specific NKCC homolog was identified based on homology cloning strategy. Second, its expression was principally studied by in situ PCR hybridization method. Subsequently, the changes of its gene expression in urinary tract obstruction (UTO) model and non-insulin dependent diabetes mellitus model (NIDDM) were studied. As a results, expression of new clone was decreased in NIDDM, suggesting that its abolished expression might generate the suppression of TGF and expose high pressure to glomerulus, causing glomerulosclerosis.
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