2002 Fiscal Year Final Research Report Summary
Meconium components affecting extracellular surfactant metabolism
| Project/Area Number |
12671068
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Saitama Medical School |
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Principal Investigator |
SHIMIZU Hiroshi Saitama Medical School, Associate Professor, 医学部, 助教授 (90260843)
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| Co-Investigator(Kenkyū-buntansha) |
OBATA Makoto Saitama Medical School, Assistant, 医学部, 助手 (70286045)
ARAKAWA Hiroshi Saitama Medical School, Assistant Professor, 医学部, 講師 (90271238)
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| Project Period (FY) |
2000 – 2002
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| Keywords | Pulmonary surfactant / Surfactant subtype conversion / Surface area cycling / Meconium / Meconium aspiration syndrome / Cellar membrane damage |
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| Research Abstract |
Large surfactant aggregates (LA) is produced and secreted by alveolar type II cells. The surface-active LA is converted to surface-inactive small surfactant aggregates (SA) after lowering surface tension at the air-liquid interface in alveoli. In the present study, we developed in vitro surface area cycling technique, a piglet model of meconium aspiration syndrome, and 1st dimension thin layer chromatography to determine surfactant phospholipid composition. Then we demonstrated that surfactant subtype conversion was accelerated under the presence of meconium in vitro and in vivo. In addition, we demonstrated a diisopropylfluorophosphate (DFP)-binding protein in meconium with an apparent molecular mass of 30 kDa. Changes of surfactant phospholipid with surfactant subtype conversion under the presence of meconium suggested phospholipase A2 activity in meconium. Cell membrane damage due to meconium was also demonstrated in this study using red blood cell model. In conclusion, meconium affected extracellular surfactant metabolism and cell membrane damage of red blood cells. The increased rate of conversion by meconium may be responsible for surfactant dysfunction that occurs with lung injuries of meconium aspiration syndrome.
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Research Products
(43 results)
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[Publications] 武内可尚, 長秀男, 山下行雄, 御宿百合子, 中尾歩, 麻生泰二, 仁志田博司, 高橋尚人, 佐久間泉, 川上義, 余田仁志, 麦島秀雄, 湊通嘉, 田口順教, 嶋田優美, 武隈孝治, 武井章, 高見剛, 小川雄之亮, 清水 浩, 高田栄子, 板橋家頭夫, 板倉敬乃, 中村利彦, 楠田聡, 金太章, 宍田紀夫, 宮城伸浩: "早産児および慢性肺疾患児におけるpalivizumabの安全性および薬物動態の検討"日本化学療法学会雑誌. 50・4. 215-222 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yoshinao Takeuchi, Hideo Cho, Yukio Yamashita, Yuriko Mishiku, Ayumi Nakao, Taiji Aso, Hiroshi Nishida, Naoto Takahashi, Izumi Sakuma, Tadashi Kawakami, Hitoshi Yoda, Hideo Mugishima, Michiyoshi Minato, Masanori Taguchi, Masami Shimada, Koji Takekuma, Akito Takei, Tsuyoshi Takami, Yunosuke Ogawa, Hiroshi_Shimizu, Eiko Takada, Kazuo Itabashi, Yukino Itakura, Toshihiko Nakamura, Satoshi Kusuda, Tashou Kim, Norio Shishida, Nobuhiro Miyagi: "Safety and pharmacokinetics of palivizumab, administered in infants with a history of prematurity or chronic lung disease (in Japanese)."Jpn J Chemotherapy. 50. 215-222 (2002)
Description
「研究成果報告書概要(欧文)」より
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