Usefulness of machine perservation on the liver transplantation from non-heart-beating donor

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671177
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Tokyo Medical University
• Principal Investigator: UCHIYAMA Masami Tokyo Medical University, Medicine, Lecuturer, 医学部, 講師 (40276902)
• Co-Investigator(Kenkyū-buntansha): KIKUCHI Kenji Tokyo Medical University Medicinem, Assistant, 医学部, 助手 (10267586) ; MATSUNO Naoto Tokyo Medical University, Medicinem, Lecture, 医学部, 講師 (00231598) ; NAGAO Takeshi Tokyo Medical University Medicinem Professor, 医学部, 教授 (90143487)
• Project Period (FY): 2000 – 2001
• Keywords: Liver transplantation / Non-heart-beating donor / Machine perfusion preservation

Research Abstract

Introduction. The shortage of organ donors is a unversal problem. We report in this study usefulness of preservation method by machine purfusion on the liver transplantation from non-heart-beating(NHB) donor after the shock state. Materials and methods. We carried out orthotopic liver transplantation(OLT) using pigs. The liver was harvested from NHB donor after 60 minutes of shock state. The grafts were preserved for 120 minutes by continuous machine perfusion via hepatic artery (8℃, modified Belzer's gluconale solution, n=20). The blood chemistry and perfusion solution chemistry after preservation were compared. And graft vascular resistance were measured at the end of preservation. esults. We evaluated twenty grafts and the recipients. It is the positive correlation between serum AST of recipient and AST in preservation solution. (Y=532.5+0.83X, =0.64, p<0.01And positivecorrelation is between serum AST level of recipient and the vascular resistance.(Y=153.3+2.36X, =0.84, p<0.01) Conclusion Machine perfusion preservation through hepatic artery allows the optimum graft condition in liver transplantation from NHB donor.