The effect of Heparin against to carcinomas which express fibronectin.

2002 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671179
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Jikei University School of Medicine
• Principal Investigator: TAKEYAMA Hiroshi Jikei University School of Medicine, Medicine Suegery Instnictotr, 医学部, 講師 (70236511)
• Co-Investigator(Kenkyū-buntansha): SAITO Yuji Jikei University School of Medicine, Medicine Suegery Fellow, 医学部, 助手 (30256383)
• Project Period (FY): 2000 – 2002
• Keywords: Heparin / Apoptosis / Gastric Carcinoma / Breast Carcinoma / Thyroid Carcinoma

Research Abstract

[Purpose]
We investigated the role of heparin binding domein of fibronectin expressing around carcinoma cells, using several carcinoma cell lines,mainly,gastric cell line (STKM) and sodium heparin.
[Materials and Methods]
1) Immunocytochemistry and flowcytometry has been done to STKM cells to confirm the expression of FN.
2) Sodium heparin was added at the concentration of 10, 50,100,200,300 IU/ml to STKM cells. The cells with heparin were investigated cell adhesion by colonmetnc assay,and cell death by thyimdine incorporated assay.
3) The monoclonal antibody against c-terminal heparin binding site of FN, IST-land IST-2 were added to STKM cells and assayed by colorimetnc, and thymidin incorporate methods.
4) bcl-2, and bcl-xl gene were transfected to STKM cells by lipofectin method, and added heparin.The transfected cells with heparin were also assayed by colorimetnc, and thymidin incorporate methods.
[Results]
1 Heparin induces apoptosis via heparin binding sites of FN, which is expressed around carcilnoma cells
2 The results of experiment, using monoclonal antibodies against heparin binding site suggest that the apoptotic effects by heparin is not related to anticoagulant effect of heparin.
3 The apoptosis induced heparin causes via mitochondria inner cells.