2002 Fiscal Year Final Research Report Summary
Gene analysis in gastric cancer using with laser captured micro-dissection
| Project/Area Number |
12671209
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | Saitama Medical School (2001-2002)
Tokyo Medical and Dental University (2000) |
|---|
Principal Investigator |
ICHIKAWA Wataru Saitama Medical School Medicine Lecturer, 医学部, 講師 (70282738)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAYAMA Renzo Saitama Medical School Medicine Professor, 医学部, 教授 (10014317)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Keywords | LCM / Cancer / TS / DPD |
|---|
| Research Abstract |
In this study, gene analysis in gastric and colorectal cancer using with laser-captured microdissection to avoid the contamination of normal interstitial tissues.
1) Cyclooxygenase-2 (COX-2):
Level of COX-2 mRNA expression in the colorectal adenomas were correlated with their size, and no relationships were demonstrable between COX-2 expression and adenoma location and degree of dysplasia.
2) Thymidine phosphorylase (TP):
No relationship between TP expression and clinicopathological features was observed in colorectal cancer. However, the prognosis of patients with TP positive expression in both cancer and stroma was worse.
3) Thymidylate synthase (TS) and Dihydropyrimidine dehydrogenase (DPD):
TS and DPD gene expressions were different among primary colorectal tumors and metastatic tumors, such as hepatic metastasis. Patients with tumors of low TS and low DPD responded to fluorouracil based chemotherapy.
In conclusion, gene analysis with laser-captured microdissection is the useful method.
|
Research Products
(4 results)
-
-
-
[Publications] Shirota, Y., Ichikawa, W., Uetake, H., Yamada, H., Nihei, Z., and Sugihara, K.: "Intratumoral dihydropyrimidine dehydrogenase messenger RNA level reflects tumor progression in human colorectal cancer"Ann Surg Oncol. 9. 599-603 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Ichikawa, W., Uetake, H., Shirota, Y., Yamada, H., Nishi, N., Nihei, Z., Sugihara, K., and Hirayama, R.: "Combination of Dihydropyrimidine Dehydrogenase and Thymidylate Synthase Gene Expressions in Primary Tumors as Predictive Parameters for the Efficacy of Fluoropyrimidine-based Chemotherapy for Metastatic Colorectal Cancer"Clin Cancer Res. 9. 786-791 (2003)
Description
「研究成果報告書概要(欧文)」より
