| Co-Investigator(Kenkyū-buntansha) |
RIKITAKE Kazuhisa Saga University, Faculty of scheschemic Medicme, Assistant, 医学部, 助手 (80295047)
ITOH Tsuyoshi Saga University, Faculty of scheschemic Medicme, Professor, 医学部, 教授 (10110496)
OKAZAKI Yukio Saga University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (80203975)
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| Research Abstract |
Objective : We examined the usefulness of leukocyte depleted filter, protease inhibitor, elastase inhibitor, or ATP sensitive potassium channel opener(PCO) in ischemic-reperfusion injury.
Methods, Experimental model was established with rabbit Langendorf preparation. Developed pressure was measured at the constant left ventricular end-diastolic pressure using the method of Latex-Baloon. Baseline control left ventricular function was measured at 15, 30, 45 and 60 minute after reperfusion following 4 hour cardioplegic arrest by St Thomas Solution. Coronary endothelial damage was observed with scanning electron microscope. Reperfusion methods were performed by leukocyte depleted i.iter, ulinastatin, protease inhibitor, elastase inhibitor, or PCO treatment. Results, Severe coronary endothelial damage was observed in control group. Coronary endothelial amage was not observed in reperfusion method by leukocyte depleted filter. Mild endothelial damages were observed in pharmacologically treated group. Postoperative cardiac function of left ventricular developed pressure at 60 minute after reperfusion was recovered by 45% in control non-treated group, by 80% in leukocyte depleted filter, by 70% in ulinastatin group, by 70% in protease inhibitor, by 78% in elastase inhibitor and by 75% in PCO treatment.
Conclusion, Potassium channel opener was excellently effective in the prevention for ichemic-reperfusion injury.
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