Gene Expresion of Soft Tissue Sarcoma

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12671404
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Shinsyu University
• Principal Investigator: GOMYO Hiroki Shinshu university, medical department, assistant, 医学部, 助手 (70311694)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Tominaga Shinshu university, medical department, assistant, 医学部・附属病院, 助手 (40283270) ; TAKAOKA Kunio Shinshu university, medical department, professor, 医学部, 教授 (30112048)
• Project Period (FY): 2000 – 2001
• Keywords: gene / gene expression / sarcoma / orthopedics

Research Abstract

Expression profiling and cluster analysis of bone and soft tissue tumors using cDNA expressin arrays.
Bone and soft tissue sarcoma are diverse in their natural history and in responsiveness in treatments. Here we have characterized variation in gene expression patterns in set of 44 surgical specimens of human bone and soft tissue tumors from 29 individuals using complementary DNA microarrays represent 1176 human genes. We analyzed gene expression patterns in grossly dissected normal or malignant human soft tissues from the same individual. We also analyzed gene expression patterns in before and after chemotherapy, and two tumors were paired with lung metastasis from the same patients. Gene expression patterns in two tumor samples from same individual were also always more similar to each other than either was to any other sample. And, we obtained 6 genes that were high expression and 27 were low expression. But these tumors could not be classified into subtypes distinguished by differences in their gene expression patterns.

Research Products

• [Publications] Seki H, Takaoka K: "Mutation frequencies of EXT1 and EXT2 in 43 Japanese families with hereditary multiple exostoses"Am J Med Genet. 15;99(1). 59-62 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Mehdi R, Gomyo H.: "Expression of bone morphogenctic protein and its receptors in osteosarcoma and malignant fibrous histiocytoma"Jpn J Clin Oncol. Jun;30(6). 272-275 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Muroya K, Gomyo H: "Sex-determining gene(s) on distal 9p : clinical and molecular studies in six cases"J Clin Endocrinol Metab. 85(9). 3094-3100 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Seki H, Kubota T, Ikegawa S, Haga N, Fujioka F, Ohzeki S, Wakui K, Yoshikawa H, Takaoka K, Fukushima Y: "Mutation frequencies of EXT1 and EXT2 in 43 Japanese families with hereditary multiple exostoses"Am J Med Genet. 99(1). 59-62 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Muroya K, Okuyama T, Goishi K, Ogiso Y, Fukuda S, Kameyama J, Sato H, Suzuki Y, Terasaki H, Gomyo H, Wakui K, Fukushima Y, Ogata T: "Sex-determining gene(s) on distal 9p clinical and molecular studies in six cases."J Clin Endocrinol Metab. 85(9). 3094-3100 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Mehdi R, Shimizu T, Yoshimura Y, Gomyo H, Takeoka K: "Expression of bone morphogenetic protein and its receptors in osteosarcoma and malignant fibrous histiocytoma."Jpn J Clin Oncol. 30(6). 272-5 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Gomyo H, Arai Y, Tanigami A, Murakami Y, Hattori M, Hosoda F, Arai K, Aikawa Y, Tsuda H, Hirohashi S, Asakawa S, Shimizu N, Soeda E, Sakaki Y, Ohki M: "A2-Mb sequence-ready contig map and immunoglobulin superfamily gene IGSF4 in the LOH region of chromosome 11q23.2"Genomics. 62(2). 139-46 (1999)
  • Description: 「研究成果報告書概要(欧文)」より