2001 Fiscal Year Final Research Report Summary
Gene TheraphyforSpinal Cord Injury
| Project/Area Number |
12671407
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUYAMA Yukihiro University Hospital, Nagoya University, Assistant Professor, 医学部・附属病院, 講師 (20312316)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Hiroshi University Hospital, Nagoya University, Medical Staff, 医学部・附属病院, 医員
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| Project Period (FY) |
2000 – 2001
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| Keywords | spinal cord injury / GDNF / microglia / macrophage |
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| Research Abstract |
Elect of hyperbaric oxygen therapy on iNOand GDNF in the acute phase of spinal cord injury.
To study the acute effect of hyperbaric oxygen ventilation (HBO) on spinal cord injury.We examined GDNFmRNA and iNOSmRNA using Reverse transcriptase-polymerase chain reaction (RT-PCR).HBO was effective within 30minitsu after spinal cord injury but the HBO after 30minitsu, instead of the times, was not effective.
Up-regulation of glial cell line-derived neurotrophic factor(GDNF) following traumatic spinal cord injury.
We investigated the temporal and spatial expression patterns of the GDNF gene after subjecting rats to an acute contusion injury of the spinal cord using the weight drop technique Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that GDNF transcription in the spinal cord began to increase within 30 min after injury and peaked within 3h. Immunohistochemical analysis showed GDNF immunoreactivity to be present mainly in microglia and acrophages 1 day after injury, but not in the neurons or astrocytes. This immediate upreguration of GDNF gene expression may be a component of an inflammatory process and probably exerts aprotective effect on neurons following apinal cord injury(SCI).
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Research Products
(20 results)
