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2002 Fiscal Year Final Research Report Summary

The larynx and Androgen, the specific receptor and the expression of the related genes

Research Project

Project/Area Number 12671686
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionSt. Marianna University, School of Medicine

Principal Investigator

URUSHIBARA Tamotsu  St. Marianna Univ., School of Med., Assistant Prof., 医学部, 講師 (70130987)

Project Period (FY) 2000 – 2002
Keywordsthe larynx / sex steroids / cancer cell / cell death / receptor gene / transcriptional coactivator / cell culture / in vivo study
Research Abstract

The larynx is an androgen target as a secondary sexual organ ; laryngeal epithelial cells have an affinity for androgen in the cell nucleus. Cancer of the larynx is the fourteenth most common incident cancer throughout the world. An estimated 190, 000 new cases were diagnosed worldwide (WHO, 1997), accounting for 1.8% of all new cancers. Men comprise 85% of the worldwide incidence of laryngeal cancer.
In this study, the laryngeal carcinoma cell line (HEp-2) was used to examine the relation of the laryngeal cancer to androgen. Two days after the treatment of 10^<-6>M testosterone propionate (Tp), the cell numbers increased, but thereafter decreased and greater than 95% of the cells died within three days, bearing no relation to cell-to-cell contact inhibition. As well as androgen, estrogen is also efficient for inducing HEp-2 cell death, however, its required concentration is one order higher (>10^<-5>M) in comparison with the tp's, but progesterone, corticosterone and cortisone are not. … More The RT-PCR products suggested that the HEp-2 cell has a faculty for transcripting the androgen receptor (AR), aromatase and estrogen receptor (ER)- β genes, but not the 5 α -reductase-I, -II, ER- α and 17 β-hydroxyrase I〜III genes. The anti-sense oligo-nucleotide transfection study suggested that this cell death needs the newly transcribing AR gene. The Tp triggers a distinct induction of SRC but the barely detectable P53 mRNA, despite the fact of marked augmentation of the P53 protein level (Tp : Control = 1242.1 : 96.6 pg/mg.p). There is no clear distinction upon transcription of the AR, HPV18-E6, -E7 and CBP genes between the Tp-treated and non-treated cells, while there is no transcription of the Rb, caspase-3, and HPV18-E2 and HPV16-E6 genes.
Taken together, these results imply that androgen may interfere with the physiology of laryngeal cancer cells, depending upon the new transcription of the AR gene and some metabolism of androgen to estrogen under the constant presence of durable p53 proteins and HPV18 in some cases. Less

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 漆畑 保: "ステロイドホルモンによるマウス移植腫瘍の成長と宿主生存期間への影響"ホルモンと臨床・冬季号. 48. 108-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 漆畑 保: "人喉頭癌細胞を用いた男性ホルモンの代謝及び活性型"ホルモンと臨床・冬季号. 49. 184-191 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamotsu Urushibata, et al.: "Castration causes unbalance of sex-steroid hormone feedback system and cancer"ITE letters. 13・4. 32-38 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 漆畑 保: "去勢が誘引となるマウス腫瘍発生と男性ホルモン感受性"ホルモンと臨床・冬季号. 50. 1105-1109 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tamotsu Urushibata: "Efficacy of steroids on the cancer growth and life span of tumor-bearing mice"Hormone and Medicine (Japanese Journal). 48. 108-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamotsu Urushibata: "The laryngeal epithelium and male steroid : the metabolism and active form"Hormone and Medicine (Japanese Journal). 49. 184-191 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamotsu Urushibata, Masako Osakabe, Tomoko Watanabe and Tsuneo Igarashi: "Castration causes unbalance of sex-steroid feedback system and cancers"ITE Letters. 13-4. 32-38 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tamotsu Urushibata, Masako Osakabe: "Murine tumors caused by castration and the androgen sensitivity"Hormone and Medicine (Japanese Journal). 50. 1105-1109 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2004-04-14  

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