2001 Fiscal Year Annual Research Report

口腔の感覚と運動発現に関与する三叉神経ニューロンの活動調節機構の機能形態学的研究

Research Project

Project/Area Number	12671768
Research Institution	Osaka University
Principal Investigator	竹村元秀大阪大学, 大学院・歯学研究科, 講師 (70192169)
Co-Investigator(Kenkyū-buntansha)	永瀬佳孝北海道医療大学, 歯学部, 講師 (50252698) 米原典文大阪大学, 大学院・歯学研究科, 助教授 (70124534)
Keywords	三叉神経 / 口腔 / 顔面 / 疼痛 / c-Fos / アンチセンス / 一酸化窒素合成酵素 / 大脳皮質
Research Abstract	ラット咬筋を切断した後の三叉神経感覚核・運動核におけるnicotinamide adenine dinucleotide phosphate-diaphorase(NADPH-d)及び神経型一酸化窒素合成酵素(n-NOS)の発現様式をそれぞれ酵素組織化学法及び免疫組織化学法を用いて時間経過を追って調べた。NADPH-dは三叉神経中脳路核では切断側に8週にわたる、運動核では両側性に一時的な新たな発現を認めた。三叉神経吻側亜核及び尾側亜核では両側性の一時的な発現の増加を認めた。n-NOSの発現はNADPH-dの発現と同様な経過を示したが、1週間遅れた。 c-Fosは痛み刺激に反応して脊髄/延髄の後角の浅層部ニューロンの核内に多く発現する転写調節因子である。c-Fosは多くのペプチドの転写を調節すると考えられているが、痛みの感覚をいかに調節するか不明である。アジュバントをラット後肢に注射して痛覚過敏を起こした後に発現するc-Fos発現をアンチセンス法を用いてブロックし、熱疼痛受容感覚の変化をプランターテストを利用して調べた。c-fosのmRNAに対するアンチセンス(AS)オリゴデオキシヌクレオチド(ODN)を腰髄に注入し4時間後アジュバントをラット後肢に注射した。AS ODN注入例ではアジュバント注射3時間後の脊髄後角のc-Fos発現をミスマッチ(MM)ODN注入例に比べ有意に減少した。さらにAS ODN注入例ではMM ODN注入例に比べ有意に熱疼痛閾値を増加した。従ってアジュバント注射により誘導される脊髄後角のc-Fosは疼痛感覚を維持したり強めたりするような転写を促進すると考えられる。さらに第1次(SI)及び第2次(SII)大脳皮質体性感覚領野を電気刺激し、延髄後角におけるc-Fosの発現様式を調べ、SIIの電気刺激が口腔領域の疼痛受容感覚を減少させることを明らかにした。

Research Products

(15 results)

All Other

All Publications (15 results)

[Publications] Gojyo, F. et al.: "Effects of somatosensory cortical stimulation on expression of c-Fos in rat medullary dorsal horn in response to formalin-induced noxious stimulation"J. Neurosci. Res.. (in press). (2002)
[Publications] Varathan, V. et al.: "Nitric oxide synthase/nicotinamide adenine dinucleotide phosphate-diaphorase in the brainstem trigeminal nuclei after transection of the masseteric nerve in rats"J. Neurosci. Res.. 66. 428-438 (2001)
[Publications] Sugiyo, S. et al.: "Effects of intrathecal c-Fos antisense olidodeoxynucleotide on adjuvant-induced thermal hyperalgesia"Exp. Brain Res.. 140. 198-205 (2001)
[Publications] Kuroda, R. et al.: "Secondary somatosensory cortex stimulation facilitates the antinociceptive effect of the NO synthase inhibitor through suppression of spinal nociceptive neurons in the rat"Brain Res.. 903. 110-116 (2001)
[Publications] Yonehara, N. et al.: "Influence of painful chronic neuropathy on neurogenic inflammation"Pain. 92. 259-265 (2001)
[Publications] Asahi, Y. et al.: "Involvement of GABAergic systems in manifestation of pharmacological activity of desipramine"Jpn. J. Pharmacol.. 86. 316-322 (2001)
[Publications] Yonehara, N. et al.: "Influence of serotonin receptor antagonists on substance P and serotonin release evoked by tooth pulp stimulation with electro-acupuncture in the trigeminal nucleus caudalis of the rabbit"Neurosience Res.. 40. 45-51 (2001)
[Publications] Yoshida, A. et al.: "Quantitative analysis of synaptic contacts made between functionally identified oralis neurons and trigeminal motoneurons in cat"J. Neurosci. 21・16. 6298-6307 (2001)
[Publications] Yoshimura, M. et al.: "Interaction between nitric oxide and substance P on heat-induced inflammation in rat paw"Pharmacol Res.. 44. 105-111 (2001)
[Publications] Takemura, M. et al.: "GABA_B receptor-mediated effects on expression of c-Fos in rat trigeminal nucleus following high-and low-intensity afferent stimulation"Neuroscience. 103. 1051-1058 (2001)
[Publications] Takemura, M. et al.: "GABA_A receptor-mediated effects on expression of c-Fos in rat trigeminal nucleus following high-and low-intensity afferent stimulation"Neuroscience. 98. 325-332 (2000)
[Publications] Takemura, M. et al.: "Mapping of c-Fos in the trigeminal sensory nucleus following high-and low-intensity afferent stimulation in the rat"Exp. Brain Res.. 130. 113-123 (2000)
[Publications] Kuroda, R. et al.: "Somatosensory cortex stimulation-evoked analgesia in rats : potentiation by NO synthase inhibition"Life Sci.. 66. 271-276 (2000)
[Publications] Yonehara, N. et al.: "Interaction between nitric oxide and substance P on heat-induced inflammation in rat paw"Neurosci. Res.. 36. 35-43 (2000)
[Publications] Yoshimura, M. et al.: "Effects of topically applied capsaicin cream on neurogenic inflammation and thermal sensitivity"Jpn. J. Pharmacol.. 82. 116-121 (2000)

2001 Fiscal Year Annual Research Report

口腔の感覚と運動発現に関与する三叉神経ニューロンの活動調節機構の機能形態学的研究

Principal Investigator

竹村 元秀 大阪大学, 大学院・歯学研究科, 講師 (70192169)

Research Products

[Publications] Gojyo, F. et al.: "Effects of somatosensory cortical stimulation on expression of c-Fos in rat medullary dorsal horn in response to formalin-induced noxious stimulation"J. Neurosci. Res.. (in press). (2002)

[Publications] Varathan, V. et al.: "Nitric oxide synthase/nicotinamide adenine dinucleotide phosphate-diaphorase in the brainstem trigeminal nuclei after transection of the masseteric nerve in rats"J. Neurosci. Res.. 66. 428-438 (2001)

[Publications] Sugiyo, S. et al.: "Effects of intrathecal c-Fos antisense olidodeoxynucleotide on adjuvant-induced thermal hyperalgesia"Exp. Brain Res.. 140. 198-205 (2001)

[Publications] Kuroda, R. et al.: "Secondary somatosensory cortex stimulation facilitates the antinociceptive effect of the NO synthase inhibitor through suppression of spinal nociceptive neurons in the rat"Brain Res.. 903. 110-116 (2001)

[Publications] Yonehara, N. et al.: "Influence of painful chronic neuropathy on neurogenic inflammation"Pain. 92. 259-265 (2001)

[Publications] Asahi, Y. et al.: "Involvement of GABAergic systems in manifestation of pharmacological activity of desipramine"Jpn. J. Pharmacol.. 86. 316-322 (2001)

[Publications] Yonehara, N. et al.: "Influence of serotonin receptor antagonists on substance P and serotonin release evoked by tooth pulp stimulation with electro-acupuncture in the trigeminal nucleus caudalis of the rabbit"Neurosience Res.. 40. 45-51 (2001)

[Publications] Yoshida, A. et al.: "Quantitative analysis of synaptic contacts made between functionally identified oralis neurons and trigeminal motoneurons in cat"J. Neurosci. 21・16. 6298-6307 (2001)

[Publications] Yoshimura, M. et al.: "Interaction between nitric oxide and substance P on heat-induced inflammation in rat paw"Pharmacol Res.. 44. 105-111 (2001)

[Publications] Takemura, M. et al.: "GABA_B receptor-mediated effects on expression of c-Fos in rat trigeminal nucleus following high-and low-intensity afferent stimulation"Neuroscience. 103. 1051-1058 (2001)

[Publications] Takemura, M. et al.: "GABA_A receptor-mediated effects on expression of c-Fos in rat trigeminal nucleus following high-and low-intensity afferent stimulation"Neuroscience. 98. 325-332 (2000)

[Publications] Takemura, M. et al.: "Mapping of c-Fos in the trigeminal sensory nucleus following high-and low-intensity afferent stimulation in the rat"Exp. Brain Res.. 130. 113-123 (2000)

[Publications] Kuroda, R. et al.: "Somatosensory cortex stimulation-evoked analgesia in rats : potentiation by NO synthase inhibition"Life Sci.. 66. 271-276 (2000)

[Publications] Yonehara, N. et al.: "Interaction between nitric oxide and substance P on heat-induced inflammation in rat paw"Neurosci. Res.. 36. 35-43 (2000)

[Publications] Yoshimura, M. et al.: "Effects of topically applied capsaicin cream on neurogenic inflammation and thermal sensitivity"Jpn. J. Pharmacol.. 82. 116-121 (2000)

竹村元秀大阪大学, 大学院・歯学研究科, 講師 (70192169)