2001 Fiscal Year Final Research Report Summary
GENERATION OF REACTIVE OXYGEN SPECIES BY CANDIDA ALBICANS : DEVELOPMENT OF AN ASSAY METHOD AND ITS APPLICATION
| Project/Area Number |
12671788
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | THE NIPPON DENTAL UNIVERSITY |
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Principal Investigator |
NAKAMURA Kenjirou THE NIPPON DENTAL UNIVERSITY, SCHOOL OF DENTISTRY AT NIIGATA, LECTURER, 新潟歯学部, 講師 (00227894)
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| Co-Investigator(Kenkyū-buntansha) |
KUWA Shoko (ITO Shoko) THE NIPPON DENTAL UNIVERSITY, SCHOOL OF DENTISTRY AT NIIGATA, LECTURER, 新潟歯学部, 講師 (40095063)
AOKI Shigeji THE NIPPON DENTAL UNIVERSITY, SCHOOL OF DENTISTRY AT NIIGATA, PROFESSOR, 新潟歯学部, 教授 (20095045)
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| Project Period (FY) |
2000 – 2001
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| Keywords | CANDIDA ALBICANS / RESPIRATION / REACTI VE OXYGEN SPECIES / SUPEROXIDE / OXIDATIVE STRESS / CHEMILUMINESCENCE |
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| Research Abstract |
Candida albicans is a predominant causative agent of mycoses in humans. This fungus, like as other aerobic organisms, is considered to generate reactive oxygen species (ROS), such as superoxide radical, hydrogen peroxide and hydroxyl radical. However, there is little information on generation of ROS in Candida yeast. In the present study, we attempted to develop a chemiluminescence method for j measuring ROS generated by C. albicans. Then, we applied the method to analyze superoxide (SOX) generation and to reveal involvement of oxidative damage in antifungal action of amphotericin B (AmB).
1. Development of a chemiluminescence method
Among some cheminescence proves tested, methyl-Cypridina-luciferin analogue (MCLA) was shown to an efficient probe for measuring SOX generated by candidal cells.
2. Relationship between respiration and superoxide generation
Endogenous SOX generation by candidal cells was very low. However, the herbcide paraquat (PQ) simulated SOX generation potently. The results obtained with a respiratory mutant, inhibitors and carbon sources indicated that the SOX generation was dependent on respiration.
3. Enhancement of AmB activity by SOX
AmB activity on growth, lethality and cell membrane of C. albicans was enhanced by PQ-induced SOX. These results indicated involvement of oxidate damage in the antifungal activity of AmB.
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Research Products
(2 results)
