2002 Fiscal Year Final Research Report Summary
Studies on the production and practical use of engineered human salivary type cystatins.
| Project/Area Number |
12671817
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | The Nippon Dental University |
|---|
Principal Investigator |
SAITOH Eiichi The Nippon Dental University School of Dentistry at Niigata Department of Biochemistry, Associate Professor, 新潟歯学部, 助教授 (40120662)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Keywords | Saliva / Human cystatin / Porphyromonas gingivalis / Cvsteine proteinases / Recombinant cystatins / Cystatin superfamily / Reverse zymography / Defense molecules |
|---|
| Research Abstract |
This research project focused on the viewpoint of practical use of biological activities of human salivary cystatins and natural cysteine proteinase inhibitors from plants and animals. In addition, a system enabling large-scale production of engineered cystatins at a Ievel of several hundreds to several thousands milligrams was investigated. Based on these concepts and plans, novel cysteine proteinase inhibitors were screened as well by the reverse zymography developed in this study. The results obtained are summarized as follows: (1) Recombinant cystatins produced by E. coli were demonstrated to induce interleukin-6 and interleukin-8 productions in the human gingival fibroblast cells via cell surface molecules. (2) The engineered salivary type cystatins were also found to depress the formation of axillary odors by microorganisms such as Brevibacterium. (3) Some experimental data have indicated that there are some components mat inhibit the growth of Porphyromonas gingivalis in the hea
… More
t-extract of the rice bran of Niigata-Koshihikari. At present, we have been studying not only oryzacystatins with molecular weight of 13 kDa but also several candidate proteins of 50-70 kDa that tightly bind to a cysteine proteinase, papain and ficin. The detail features of the substances are now under investigation by the reverse zymographical technique since rice cysteine proteinase inhibitors could be a potent inhibitor of the growth of P. gingivalis as well as salivary cystatins. (6) The mechanism of a defense system present in the skin mucus of fishes may be helpful to study physiological roles of salivary proteins in oral cavity. Hence, we have attempted to search for flie defense proteins such as proteainses, proteinase inhibitors, and the lectins in the mucus fluid of the Japanese eel. As the result, the skin mucus was shown to contain novel cysteine proteinase inhibitor, with molecular masses of about 13-15 kDa. However, it is obscure at present that the inhibitors may be the members of cystatin superfamily, or not. Taken together, it can be concluded that cysteine proteinase inhibitors from plants and animals are thought to be a considerably useful biomaterial for the maintenance of oral health and for the clinical trials of periodontitis. Less
|
Research Products
(15 results)
-
[Publications] Kato T., Imatani, T., Miura, T., Minaguchi, K., Saitoh, E., Okuda, K.: "Cytokine inducing activity of family 2 cystatins"Biological Chemistry. Vol.381. 1143-1147 (2000)
Description
「研究成果報告書概要(和文)」より
-
-
-
-
[Publications] Saitoh, E., Oka, K., Isemura, S., Chiba, A., Odani, S.: "Fractionation and characterization of cysteine protease inhibitors in the mucus fluid of Japanese eel (Anguilla Japonica)"Abstracts of 3^<rd> International Conference on Cysteine Proteinases and Their Inhibitors (Turk, V., ed). 107 (2002)
Description
「研究成果報告書概要(和文)」より
-
[Publications] Oka, K., Saitoh, E., CHiba, A., Isemura, S., Sanada, K.: "Characterization of proteins and proteases in the mucus fluid of Japanese eel (Anguilla Japonica)"Abstracts of 3^<rd> International Conference on Cysteine Proteinases and Their Inhibitors (Turk, V., ed.). 64 (2002)
Description
「研究成果報告書概要(和文)」より
-
[Publications] Kato, T., Imatani, T., Miura, T., Minaguchi, K., Saitoh, E. & Okuda, K.: "Cytokine-inducing activity of family 2 cystatins."Biological Chemistry. Vol.381No.11. 1143-1147 (2000)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Minaguchi, K., Kiriyama, T., Saitoh, E., Isemura, S., & Sanada, K.: "Sac I restriction fragment length polymorphism (RFLP) related to the hidnan CST2 gene."The Bulletin of Tokyo Dental College. Vol.41, No.1. 41-44 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Kato, T., Imatani, T., Minaguchi, K., Saitoh, E., & Okuda, K.: "Salivary cystatins induce interleukin-6 expression via cell surface molecules in human gingiva! fibrablasts."Molecular Immunology. Vol. 39, No. 7-8. 423-430 (2002)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
[Publications] Saitoh, E., Oka, K., Isemura, S., Chiba, A, & Odani, S.: "Fractionation and characterization of eysteine protease inhibitors in the mikcus fluid of Japanese eel (Anguitta Japonica).,"107 (2002)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Oka, K., Saitoh, E., Chiba, A, Isemura, S., & Sanada, K..: "Characterization of proteins and proteases in the mucus fluid of Japanese eel (Anguilla Japonica)., Book of Abstracts of the 3rd International Conference on Cysteine Proteinases and Their Inhibitors (Turk, V., ed)"64 (2002)
Description
「研究成果報告書概要(欧文)」より
