2001 Fiscal Year Final Research Report Summary
Experimental Study of Controlled Freezing Point Strage Method(Hyo-on) on Tooth Transplantation and Replantation.
| Project/Area Number |
12671900
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Showa University |
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Principal Investigator |
TAKIZAWA Hideki Showa University, School of Dentistry, assistant professor, 歯学部, 講師 (50236387)
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| Project Period (FY) |
2000 – 2001
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| Keywords | tooth transplantation / tooth replantation / Hyo-on / UW solution / trehaloce / glycerol / DMSO |
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| Research Abstract |
An experiment was done by using peridontal ligament(P.D.L.) of beagle dogs for the purpose of the long-term preservation of tooth transplant and replantation.
First, tooth were preserved with four kinds of preservation solutions(University of Wisconsin solution, Euro-collins solution, Dulbecocos Modified Eagles medium and Isotonic sodium chloride) in 4℃. In result, UW was the best was shown for one week after the preservation as a result of looking up P.D.L.cellular activation with out-growth method.
Next, the reference of the preservation condition was done. The activation of PDLcell was looked up with out-growth method after -2℃ which was Hyo-on preservation which was -80℃ as to preservation temperature of the cell which Was 4℃ as to internal organs preservation temperature was set up and a tooth was kept in each temperature in the UW. As for the preservation example as well, P.D.L cell which kept Hyo-on in that result UW recognized cellular multiplication for 21 days.
So, tooth replantation experiment of the kept tooth was done by using beagle dogs. A preservation priod was 4＆6 weeks Presavation temperature was set up in 4℃, -80℃, -2℃. After 8weeks, dogs were slaughtered and histological examination, was done. After 4weeks preserved in Hyo-on(-2℃)tooth were replanted and it worked like healthy tooth.
The research of the freezing preservation was done to keep P.D.L. cellular multiplication for more long term. 3 kinds of freezing prevention materiaI (trehloce glycerol DMSO) were added to the preservation solutions(UW), 0% &10%. Preservation temperature was -2℃&-80℃, after preservation the analysis of the cell multiplication examination, movement Noh play examination was done. A good record was shown in the order of U.W+DMSO in -80C. UW+ glycerol and U.W+trehaloce in -80 C.
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