2003 Fiscal Year Final Research Report Summary
Genetic analysis of the polymorphic carbohydrate structures of gengliasides for nerve regeration and a novel therpeutic approach
| Project/Area Number |
12671949
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
MIZUNO Akio Nagasaki University, Graduated School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (00014267)
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| Co-Investigator(Kenkyū-buntansha) |
FURUKAWA Koichi Nagasaki University, Medicine, Professor, 医学部, 教授 (80211530)
ITO Michihiochiro Nagasaki University, Hospital of Medicine and Dentistry, Lecturer, 医学部・歯学部附属病院, 講師 (70223188)
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| Project Period (FY) |
2000 – 2003
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| Keywords | Ganglioside / Regeneration / Hypoglossal Nerve / Knockout mouse / b-series |
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| Research Abstract |
The polymorphic carbohydrate structures of gangliosides play regulatory roles. In particular, b-series gangliosides, all of which contain alpha-2,8 sialic acids, have been considered to be critical in various biological events such as adhesion, toxin binding, neurite extension, cell growth, and apoptotis. To clarify the physiological functions of b-series gangliosides in viva, we have established a gene knockout mouse of GDS syntliase. Arthough all 'o-series structures were detieted in he mutant mice, they showed an almost complete nervous tissue morphology with no apparent abnormal behavior. Moreover, no differences in Fas-mediated apoptotic reaction of lymphocytes between wild type and the mutant mice were detected. However, the mutant mice exhibited clearly 'reduced regeneration of axotomized hypoglossal nerves compared with the wild type, suggesting that b-series gangliosides are more important in the repair rather than the differentiation of the nervous system and apoptotic process induced via Fas.
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Research Products
(8 results)
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[Publications] Itoh, M., Fukumoto, S., Iwamoto, T., Mizuno, A., Rokutanda, A., Furukawa, K.: "Specificity of carbohydrate structures of gangliosides in the activity to regenerate the rat axotomized hypoglossal nerve"Glycobiology. 11. 125-130 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Itoh, M., Izumi, S., Uemura, M., Mizuno, A., Nakane, P.K., Koji, T.: "Prevention of death of axotomized hypoglossal neurones and promotion of regeneration by chitin grafting"Cellular and Molecular Neurobiology. 20. 529-540 (2000)
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[Publications] Okada, M., Itoh, M., Haraguchi, M., Okajima, T., Inoue, M., Ohishi, H., Matsuda, Y., Iwamoto, T., Kawano, T., Fukumoto, S., Miyazaki, H., Furukawa, K., Aizawa, S., Furukawa, K.: "b-series gangliosides-lacking mice showed no changes in the neurogenesis and the sensitivity to Fas-mediated apotosis, but exhibited reduced regeneration of the lesioned hypoglossal nerve."J.Bio.Chem.. 277. 1633-1636 (2002)
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「研究成果報告書概要(欧文)」より
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