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2001 Fiscal Year Final Research Report Summary

Development on practical examination methods using DNA polymorphisms in the field of Forensic Dentistry

Research Project

Project/Area Number 12672014
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 矯正・小児・社会系歯学
Research InstitutionTOKYO DENTAL COLLEGE

Principal Investigator

MANAGUCHI Kiyoshi  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, PROFESSOR, 歯学部, 教授 (00133380)

Co-Investigator(Kenkyū-buntansha) HAGA Toshiaki  TOKYO DENTAL, COLLEGE, DEPARTMENT OF DENTISTRY, ASSISTANT, 歯学部, 助手 (80287185)
HANAOKA Yoichi  TOKYO DENTAL COLLEGE, DEPARTMENT OF DENTISTRY, LECTURER, 歯学部, 講師 (30180912)
Project Period (FY) 2000 – 2001
KeywordsMaterial examination / Mitochondrial DNA / Y-chromosome polymorphism / VWA locus / STR and SNPs / DYS390 locus / Race identification / STRs on autosomal chromosomes
Research Abstract

The purpose of this study is to establish practicable and reliable methods to detect DNA polyomorphisms using highly degraded DNA sources in Forensic Dentistry. In the first year, we examined if reduction of the PC size on STs gave reproducible results and also if it improved amplification efficiency from degraded DNAs. We compared reliability of amplification of the small-sized ST loci, such as D12SG6, VWA and CD4 and examined influences on typing when the primers producing different-sized PCB products from the same polymorphic region were constructed and applied to the PC. The results indicated that reduction of the PC size increased amplification efficiency from degraded DNAs, but did not coordinate reliability of typing. We investigated sequence polymorphism of mitochondrial DNA control regions from 201 Japanese individuals and classified 165 haplotypes into 32 clusters based on the results of Neighbour Joioning analysis. Each clusters shared specific polymorphic sites, and these characteristics suggested possible application of mtDNA polymorphisms to ethnic group estimation from forensic biological samples. We also investigated 10 Y chromosome ST polymorphisms in the Japanese population and also shoed structural variation in the DYS390 locus, resulting in the identification of highly informative discriminatory power of Y-ST polymorphisms. Beauses ST polymorphisms sometimes gave erroneous results in PC of degraded DNA, we extended our studies on Y-SNPs by designing primers to amplify small PC products, which made it possible to obtain amplification products from highly degraded DNAs. Autosomal ST polymerphisms and SNPs were also examined to compare reliability of typing from degraded DNA samples. Haplotype analysis on Y-SNPs indicated the presence of several lineages in male individuals in the Japanese population. We also showed procedure and resin used by the patients.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Minaguchi, K., et al.: "Polymorphism of the D12S66 system in the Japanese population and its detection using degraded DNA"Bull Tokyo Dent Coll. 41. 15-20 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minaguchi, K., et al.: "Structural variations of the VWA locus in humans and comparison with non-human primates"Forensic Sci Int. 113. 9-16 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hayashi, K., et al.: "An autopsy case of Klinefelter's syndrome suspected and its DNA analysis"Forensic Sci Int. 113. 119-125 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Inoue, M., et al.: "Personal identification by DNA analysis of samples from dental prostheses made of acrylic resin"Bull Tokyo Dent Coll. 41. 175-185 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 宇都野 創 他: "CD4 locusのPCR増幅と増幅サイズのわずかに異なるVWA locusとの物体検査への応用の比較"DNA多型. Vol.8. 317-319 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野中 育 他: "DYS389 locusの物体検査への応用のための増幅領域の変更とプライマー設計による影響"DNA多型. Vol.8. 320-323 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 水口 清: "顎顔面領域の情報からの個人識別"LISA. Vol.7(No.8). 816-817 (2000)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nonaka, I., et al.: "Allele frequencies and haplotypes of ten Y-specific STRs in the Japanese population"J Forensic Sci. 46. 179-182 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 野中 育 他: "DYS390の塩基配列多型とSSCP/HD analysisによる多型検出および非ヒト霊長類との比較"DNA多型. Vol.9. 230-233 (2001)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minaguchi K., et al.: "Sac I restriction fragment length polymorphism (RFLP) related to the human CST2 gene"Bull Tokyo Dent Coll. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minaguchi k et al.: "Polymorphism of the DI2S66 system in the Japanese population and its detection using degraded DNA."Bull Tokyo Dent Coll. 41. 15-20 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minaguchi K et al.: "Structural variations of the VWA locus in humanas and comparison with non-human primates."Forensic Sci Int. 113. 9-16 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hayashi K et al.: "An autopsy case of Klinefelter's syndrome suspected and its DNA analysis."Forensis Sci Int. 113. 119-125 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue m et al.: "Personal identification by DNA analysis of samples from dental prostheses made of acrylic resin."Bull Tokyo Dent Coll. 41. 175-185 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Utsuno H et al.: "PCR amplification of the CD4 locus and comparison with amplification of the VWA locus when applied to material examination"DNA polymorphism. Vol.8. 317-319 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nonaka I et al.: "Allele frequencies and haplotypes of ten Y-specific STRs in the Japanese population."J Forensic Sci. 46. 179-182 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nonaka I et al.: "DYS390 locus : Sequence polymorphism, SSCP/HD analysis and the counterparts in non-human primates."DNA polymorphism. Vol.9. 230-233 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minaguchi K.: "Personal identification using information of caranio-facial regions."LISA. Vol. 7(No.8). 816-817 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minaguchi K et al.: "sac I restriction fragment length polymorphism (RFLP) related to the human CST2 gene."Bull Tokyo Dent Coll. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2003-09-17  

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