Quantitative Evaluation of Biomaterial Surfaces by Cell Differentiation

2001 Fiscal Year Final Research Report Summary

• Project/Area Number: 12672030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MASUDA Takayuki Tokyo Medical and Dental University, Fixed Prosthodontics, Research Associate, 大学院・医歯学総合研究科, 助手 (90251537)
• Project Period (FY): 2000 – 2001
• Keywords: biomaterials / surface processing / Quantitative evaluation / gene transfer / stable expression / reporter gene / bone sialoprotein / osteocalcin

Research Abstract

In order to evaluate new bone formation at the implant surfaces quantitatively, chimeric cells were created with stable transfection of promoter-reporter gene fragment of bone sialoprotein and osteocalcin. Bone sialoprotein and osteocalcin were thought to have specific relationship with new bone formation in the early stage and in the late stage respectively. Therefore, these two extracellular matrices should be enough for chronological evaluation in vitro.
First, the relationship between those cell lines and major extracellular matrices such as fibronectin, laminin, collagen I and collagen IV were evaluated with coated plastic dishes with quantatative analyses of β-galactosidase reporter expression. The results showed the cell lines recognized every surface coating and varied reporter expression. However any morphological difference between each condition was observed at light microscopic level.
Then these cell lines were incubated on the several kinds of processed cp titanium surfaces such as acid etched, glass bead blasted, alumina blasted and machined. The result showed differences in each surface processing, however, due to larger deviation of the results, it is difficult to conclude optimum processing method.