2001 Fiscal Year Final Research Report Summary
Direct analysis of microorganisms involved in the metabolism of multiple aromatic compounds and the production bound residues in soil
| Project/Area Number |
12680568
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
環境保全
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
KATAYAMA Arata Nagoya University, Research Center for Advanced Waste and Emission Management, Professor, 難処理人工物研究センター, 教授 (60185808)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Akira Nagoya University, Graduate School ofBioagricultutal Sciences, Associate Professor, 生命農学研究科, 助教授 (50231098)
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| Project Period (FY) |
2000 – 2001
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| Keywords | aromatic compounds, / microbial degradation, / dehydrogenase / dechlonnation / quinone profile / ^<14>C quinone / soil / multiple substrates |
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| Research Abstract |
This study was conducted to develop the methods (1) to analyze directly the microbial groups metabolizing multiple subtrates in soil based on ^<14>C quinone profile and (2) to analyze directly the chemical structure of bound residues produced during the degradation of aromatic compounds in soil.
(1)The microorganisms degrading phenol with the presence of plucose orbenzoic acid were determined by ^<14>C quinone profile method. The microorganisms degrading phenol without other chemicais was mainly the group containing ubiquinone-10 that is alpha-subclass of Proteobacteria. With the presence of glucose, the menaquinone-8(H4) group was the phenol degrader in addition to the ubiquinone-10 group. Menaquinone-8(H4) is contained in Corynebacterineae, Micrococcineae, Propionibacterineae and Pseudonocardineae. With the presence of benzoic acid, the major phenol degraders were not different without benzdc acid These: findmgs has not been obtained. This method enabled the detection of microorganisms degrading a organic compounds with the presence of multiple organic compounds. It will contribute to understand the microbial community in soil. Changes in chemical structures of compounds (^<13>C-labeled rice straw and (^<15>_<13>NH_4)_2SO_4) were measured using ^<13>C-CP/MAS-NMR and ^<15>N-CP/MAS-NMR. Changes in ^<13>C-carbon composition in humic acid during the decomposition of nee straw were directed to that of soil humic acid contained in the S used Changes of nitrogenous compounds were also successfully measured A part of Sen wa, incorporated into fulvic acid that has been considered as inert compounds SgTu^cfducose and a pure culture containing multi microorganisms with a large, the microorganisms degrading glucose was successfully detected. The detection limit of ^<13>C was found relatively low so that the higher labeling levels were required.
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Research Products
(16 results)
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[Publications] Mori, T., Itoh, T., Yamamoto, H., Fujie, K., Katayama, A.: "Direct isolation of fungal hyphae degrading 2,4,5,6-tetrachloroisophthalonitrile from soil"Soil Sci. Plant Nutr.. 46. 745-749 (2000)
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「研究成果報告書概要(和文)」より
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[Publications] Watanabe, A., Sarno, Rumbanraja, J., Tsutsuki, K, Kimura, M.: "Humus composition of soils under forest, coffee, and arable cultivation in hilley areas of south Sumatra, Indonesia"European J. Soil Sci. 52. 599-606 (2001)
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「研究成果報告書概要(和文)」より
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[Publications] Mori, T., Itoh, T., Yamamoto, H., Fujie, K. and Katavama. A: "Direct isolation of fongal hyphae degrading 2,4,5,6-tetrachloroisophtfaalonitrile from soil"Soil Sci. Plant Nutr. 46. 745 (2000)
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「研究成果報告書概要(欧文)」より
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[Publications] Watanabe, A. Sarno, Rumbanraja, J., Tsutsuki, K, and Kimura, M: "Humus comoosition of soils under forest, coffee, and arable cultivation in hillpv areas of south Sumatra, ndonesia"European J oil Sci. 52(4). 599 (2001)
Description
「研究成果報告書概要(欧文)」より
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