2002 Fiscal Year Final Research Report Summary
Biologically sctive substances emitted by aquatic organisms effective at the trace concentration
| Project/Area Number |
12680592
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bioorganic chemistry
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
OOI Takashi The University Tokushima, Faculty of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (00203696)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUSUMI Takenori The University Tokushima, Faculty of pharmaceutical Sciences, Professor, 薬学部, 教授 (70015882)
|
|---|
| Project Period (FY) |
2000 – 2002
|
| Keywords | Daphnia / Sceneclesmus / Kairomone / coenobium / Enteromorpha / terpene / aquatic organism |
|---|
| Research Abstract |
1. Daphnia and morphological change of green algae : In the usual cultivation condition, green algae genera Scenedesmus and Actinastrwn grow as single-cell. However, when cultivated in the presence of Daphnia the algae achieves morphological change to 2-, 4-or 8-cell coenobia. Such morphological change of the microalga is considered to be triggered by a chemical substance, a kairomone, excreted by the water flea. We found that oxidized fatty acids might be colony formation inducer of Actinastrum sp.. Consequently, it has become clear that Daphnia water does not induce the morphological change on Actinastrum sp., but it delays reversion of the coenobia to single-cells. It has been shown that 13-HODE and 9-KODE are causative of the delay. With respect to Scenedesmus sp. remarkable colony formation with Daphnia water has been observed. It has been proved drat 13-HODE and 9-KODE do not have effect at all on this alga. As a result of the search for the inducer, the fraction inducing morphological change at the concentration below 100 ppb has been obtained
2. Maturation induction inhibitor of green alga Enteromorpha prolifera : In the thallus of E.prolifera, the material, which inhibits maturation of the cell of itself, is included Separation was carried out to isolate this material ; we found that at least 2 types of inhibitor exist. One is water soluble and other is lipophilic
3. Other compounds : New terpenoid components cyclosinularane and (+)-alloaromadendrene were isolated from the volatile oil of die soft corals. The brominated indoles were isolated as components of brittle stars, and structural studies by 13C-NMR relaxation time were carried out. From the soft coral, new cembranoid was isolated
|
