2001 Fiscal Year Final Research Report Summary
Novel Factor Oxidized Galectin-1 Advances Functional Recovery after Peripheral Nerve Injury
| Project/Area Number |
12680758
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Waseda University (2001)
Yokohama City University (2000) |
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Principal Investigator |
HORIE Hidenori Waseda University, Institute for Biomedical Science, Professor, 先端バイオ研究所, 教授 (80046135)
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| Project Period (FY) |
2000 – 2001
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| Keywords | Oxidized galectin-1 / regeneration / peripheral nerve / functional recovery / injury / transection |
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| Research Abstract |
We have revealed that oxidized galectin-1 promotes axonal regeneration from transected-nerve sites in an in vitro DRG explant model as well as in vivo peripheral nerve axotomy models. The purpose of this project is to clarify whether oxidized galectin-1 advances restoration of nerve functions after peripheral nerve injury.
The sciatic nerve of an adult rat was transected and then the distal nerve was frozen after sutured into proximal site with four epineurial stitches. Peripheral delivery of oxidized galectin-1 or control solvent to the surgical site was performed with the osmotic pump. The functional recovery was evaluated by measuring the degree of toe spread of the hind paw. The recovery curves indicated that oxidized galectin-1 administration clearly advanced functional recovery (p<0.05 compared with control PBS group by ANOVA). The histological study showed that oxidized galectin-1 increased numbers of regenerating myelinated axons in a sciatic nerve after axotomy. There was the tendency that diameters of the myelinated axons were large in oxidized galectin-1 administrating group. These results indicate that administration of oxidized galectin-1 to the nerve injury sites is effective to the rapid restoration of the nerve function.
We have performed the purpose of this project during its period and are now preparing to publish a paper of this work.
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Research Products
(14 results)
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[Publications] Hirono M, Sugiyama T, Kishimoto Y, Sakai I, Miyazawa T, Kishio M, Inoue H, Nakao K, Ikeda M, Kawahara S, Kirino Y, Katsuki M, Horie H, Ishikawa Y, Yoshioka T.: "Phospholipase Cβ4 and protein kinase Cα and/or protein kinase CβI are involved in the induction of long-term depression in cerebellar purkinje cells"J. Biol Chem. 276. 45236-45242 (2000)
Description
「研究成果報告書概要(欧文)」より
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